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  • Featured Product
  • KD/KO Validated

THOC7 Polyclonal antibody

THOC7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17881-1-AP

Synonyms

fSAP24, hTREX30, NIF3L1 binding protein 1, NIF3L1BP1, THO complex subunit 7 homolog, THOC7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using THOC7 Polyclonal antibody (17881-1-AP)

    WB analysis using 17881-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17881-1-AP (THOC7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using THOC7 Polyclonal antibody (17881-1-AP)

    WB analysis of human liver using 17881-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 17881-1-AP (THOC7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using THOC7 Polyclonal antibody (17881-1-AP)

    WB analysis of HEK-293 using 17881-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17881-1-AP (THOC7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using THOC7 Polyclonal antibody (17881-1-AP)

    IHC staining of human kidney using 17881-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17881-1-AP (THOC7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using THOC7 Polyclonal antibody (17881-1-AP)

    IHC staining of human kidney using 17881-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17881-1-AP (THOC7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using THOC7 Polyclonal antibody (17881-1-AP)

    IHC staining of human testis using 17881-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17881-1-AP (THOC7 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using THOC7 Polyclonal antibody (17881-1-AP)

    IHC staining of human testis using 17881-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17881-1-AP (THOC7 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using THOC7 Polyclonal antibody (17881-1-AP)

    IHC staining of human spleen using 17881-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17881-1-AP (THOC7 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using THOC7 Polyclonal antibody (17881-1-AP)

    IHC staining of human spleen using 17881-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17881-1-AP (THOC7 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using THOC7 Polyclonal antibody (17881-1-AP)

    IF Staining of A431 using 17881-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using 17881-1-AP (THOC7 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, human liver tissue, HEK-293 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman kidney tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17881-1-AP targets THOC7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen THOC7 fusion protein Ag11795 相同性解析による交差性が予測される生物種
Full Name THO complex 7 homolog (Drosophila)
Calculated molecular weight 204 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC065012
Gene symbol THOC7
Gene ID (NCBI) 80145
RRIDAB_10548743
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

THOC7 is a member of the THO complex that associate with the mRNA export apparatus. It is required for efficient export of polyadenylated RNA. It acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production.

Protocols

Product Specific Protocols
WB protocol for THOC7 antibody 17881-1-APDownload protocol
IHC protocol for THOC7 antibody 17881-1-APDownload protocol
IF protocol for THOC7 antibody 17881-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Viruses

Murine Leukemia Virus Uses TREX Components for Efficient Nuclear Export of Unspliced Viral Transcripts.

Authors - Toshie Sakuma
  • KD Validated
View Article