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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

THOP1 Recombinant antibody

THOP1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241607G8

Cat no : 84319-3-RR

Synonyms

THOP 1, MP78, MEPD_HUMAN, EP24.15, EC:3.4.24.15

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using THOP1 Recombinant antibody (84319-3-RR)

    WB analysis using 84319-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84319-3-RR (THOP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84319-3-RR

    Affinity and Kinetic Characterization of 84319-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84319-3-RR against Human THOP1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84319-3-RR is THOP1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen THOP1 fusion protein Ag2268 相同性解析による交差性が予測される生物種
Full Name thimet oligopeptidase 1
Calculated molecular weight 689 aa, 79 kDa
Observed molecular weight75 kDa
GenBank accession numberBC000135
Gene symbol THOP1
Gene ID (NCBI) 7064
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Thimet oligopeptidase(THOP1) is a metallopeptidase implicated in the metabolism of many sleep-related peptides, including angiotensin I, gonadotropin releasing hormone (GnRH), neurotensin, and opioid peptides

Protocols

Product Specific Protocols
WB protocol for THOP1 antibody 84319-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols