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  • Featured Product
  • KD/KO Validated

THRA Monoclonal antibody, PBS Only

THRA Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A11C8

Cat no : 66703-1-PBS

Synonyms

EAR7, EAR 7, c-erbA-alpha, c-erbA-1, AR7

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using THRA Monoclonal antibody (66703-1-Ig)

    WB analysis of A431 using 66703-1-Ig (same clone as 66703-1-PBS)

    WB result of THRA antibody (66703-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-THRA transfected A431 cells. This data was developed using the same antibody clone with 66703-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using THRA Monoclonal antibody (66703-1-Ig)

    IHC staining of rat heart using 66703-1-Ig (same clone as 66703-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66703-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using THRA Monoclonal antibody (66703-1-Ig)

    IHC staining of mouse brain using 66703-1-Ig (same clone as 66703-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66703-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using THRA Monoclonal antibody (66703-1-Ig)

    IHC staining of mouse brain using 66703-1-Ig (same clone as 66703-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66703-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using THRA Monoclonal antibody (66703-1-Ig)

    IHC staining of human thyroid cancer using 66703-1-Ig (same clone as 66703-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66703-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using THRA Monoclonal antibody (66703-1-Ig)

    IHC staining of mouse heart using 66703-1-Ig (same clone as 66703-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66703-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using THRA Monoclonal antibody (66703-1-Ig)

    IHC staining of rat colon using 66703-1-Ig (same clone as 66703-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66703-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using THRA Monoclonal antibody (66703-1-Ig)

    IF Staining of A431 using 66703-1-Ig (same clone as 66703-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using THRA antibody (66703-1-Ig, Clone: 1A11C8 ) at dilution of 1:1300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66703-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66703-1-PBS targets THRA in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen THRA fusion protein Ag27189 相同性解析による交差性が予測される生物種
Full Name thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian)
Calculated molecular weight 54 aa, 7 kDa
Observed molecular weight45 kDa
GenBank accession numberBC002728
Gene symbol THRA
Gene ID (NCBI) 7067
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.