THUMPD1 Polyclonal antibody

Name: THUMPD1 Polyclonal antibody
Brand: Proteintech
SKU: 14921-1-AP

THUMPD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14921-1-AP

Synonyms

THUMPD 1, THUMP domain-containing protein 1

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 14921-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14921-1-AP (THUMPD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 14921-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 14921-1-AP (THUMPD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 14921-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 14921-1-AP (THUMPD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 14921-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14921-1-AP (THUMPD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 14921-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14921-1-AP (THUMPD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 14921-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 14921-1-AP (THUMPD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 14921-1-AP

Size/Concentration

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, K-562 cells, MCF-7 cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

14921-1-AP targets THUMPD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	THUMPD1 fusion protein Ag6739 相同性解析による交差性が予測される生物種
Full Name	THUMP domain containing 1
Calculated molecular weight	39 kDa
Observed molecular weight	49 kDa
GenBank accession number	BC000448
Gene symbol	THUMPD1
Gene ID (NCBI)	55623
RRID	AB_2271785
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for THUMPD1 antibody 14921-1-AP	Download protocol
IHC protocol for THUMPD1 antibody 14921-1-AP	Download protocol
IF protocol for THUMPD1 antibody 14921-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Death Differ Lactylation of NAT10 promotes N4-acetylcytidine modification on tRNASer-CGA-1-1 to boost oncogenic DNA virus KSHV reactivation Authors - Qin Yan View Article
human	IF	Biosci Rep Pan-cancer analysis of N4-acetylcytidine adaptor THUMPD1 as a predictor for prognosis and immunotherapy. Authors - Kuangxun Li View Article
human	IHC	iScience RNA modification-related genes illuminate prognostic signature and mechanism in esophageal squamous cell carcinoma Authors - Hui Han View Article

製品

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サポート

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

THUMPD1 Polyclonal antibody

THUMPD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 14921-1-AP

WB analysis of K-562 using 14921-1-AP

WB analysis of MCF-7 using 14921-1-AP

IHC staining of human breast cancer using 14921-1-AP

IHC staining of human breast cancer using 14921-1-AP

IF Staining of MCF-7 using 14921-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Lactylation of NAT10 promotes N4-acetylcytidine modification on tRNASer-CGA-1-1 to boost oncogenic DNA virus KSHV reactivation

Pan-cancer analysis of N4-acetylcytidine adaptor THUMPD1 as a predictor for prognosis and immunotherapy.

RNA modification-related genes illuminate prognostic signature and mechanism in esophageal squamous cell carcinoma