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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

THYN1 Monoclonal antibody

THYN1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E12G3

Cat no : 68337-1-Ig

Synonyms

HSPC144, MDS012, My0054, MY105, THY28, THY28KD, thymocyte nuclear protein 1, Thymocyte protein Thy28, THYN1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using THYN1 Monoclonal antibody (68337-1-Ig)

    WB analysis using 68337-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68337-1-Ig (THYN1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of various lysates using THYN1 Monoclonal antibody (68337-1-Ig)

    WB analysis using 68337-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68337-1-Ig (THYN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using THYN1 Monoclonal antibody (68337-1-Ig)

    IHC staining of mouse testis using 68337-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68337-1-Ig (THYN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using THYN1 Monoclonal antibody (68337-1-Ig)

    IHC staining of mouse testis using 68337-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68337-1-Ig (THYN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inLNCaP cells, U2OS cells, HeLa cells, HepG2 cells, K-562 cells, Jurkat cells, MOLT-4 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68337-1-Ig targets THYN1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen THYN1 fusion protein Ag8617 相同性解析による交差性が予測される生物種
Full Name thymocyte nuclear protein 1
Calculated molecular weight225aa,26 kDa; 166aa,19 kDa
Observed molecular weight28 kDa
GenBank accession numberBC006978
Gene symbol THYN1
Gene ID (NCBI) 29087
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

THYN1, also named as THY28, is a 28-32 kDa protein which located mainly in the nucleus. It appears to correlate with induction of apoptosis. (PMID:14601557)

Protocols

Product Specific Protocols
WB protocol for THYN1 antibody 68337-1-IgDownload protocol
IHC protocol for THYN1 antibody 68337-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols