TIA1 Monoclonal antibody
TIA1 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2C11F4
Cat no : 68486-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HUVEC cells, hTERT-RPE1 cells, HepG2 cells, COLO 320 cells, MOLT-4 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
Positive IHC detected in | mouse liver tissue, human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68486-1-Ig targets TIA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | TIA1 fusion protein Ag2778 相同性解析による交差性が予測される生物種 |
Full Name | TIA1 cytotoxic granule-associated RNA binding protein |
Calculated molecular weight | 214 aa, 24 kDa, 43 kDa |
Observed molecular weight | 38-40 kDa |
GenBank accession number | BC015944 |
Gene symbol | TIA1 |
Gene ID (NCBI) | 7072 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TIA1, also named as p40-TIA-1, is involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). It possesses nucleolytic activity against cytotoxic lymphocyte target cells. TIA1 may be involved in apoptosis. Two isoforms of this protein exist - 41kDa and 42kDa. one of these was a missense variant (P362L) in TIA1. Similar to the ALS-related disease proteins TDP-43, hnRNPA1, and FUS, TIA1 is an RNA-binding protein containing a prionlike LCD and assembles into membrane-less organelles, including SGs. Postmortem neuropathology of five TIA1 mutations carriers showed a consistent pathological signature with numerous round, hyaline, TAR DNA-binding protein 43 (TDP-43)-positive inclusions.TIA1mutations significantly increased the propensity of TIA1 protein to undergo phase transition. In live cells,TIA1mutations delayed stress granule (SG) disassembly and promoted the accumulation of non-dynamic SGs that harbored TDP-43.
Protocols
Product Specific Protocols | |
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WB protocol for TIA1 antibody 68486-1-Ig | Download protocol |
IHC protocol for TIA1 antibody 68486-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |