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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TIA1 Recombinant antibody, PBS Only

TIA1 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3N22

Cat no : 82672-1-PBS

Synonyms

RNA binding protein TIA 1, p40-TIA-1, p40 TIA 1, Nucleolysin TIA-1 isoform p40, Cytotoxic granule associated RNA binding protein TIA1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TIA1 Recombinant antibody (82672-1-RR)

    WB analysis using 82672-1-RR (same clone as 82672-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82672-1-RR (TIA1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82672-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using TIA1 Recombinant antibody (82672-1-RR)

    WB analysis of Jurkat using 82672-1-RR (same clone as 82672-1-PBS)

    WB result of TIA1 antibody (82672-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TIA1 transfected Jurkat cells. This data was developed using the same antibody clone with 82672-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TIA1 Recombinant antibody (82672-1-RR)

    IF Staining of HeLa using 82672-1-RR (same clone as 82672-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using TIA1 antibody (82672-1-RR, Clone: 3N22 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 82672-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TIA1 Recombinant antibody (82672-1-RR)

    IF Staining of HeLa using 82672-1-RR (same clone as 82672-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using TIA1 antibody (82672-1-RR, Clone: 3N22 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82672-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82672-1-PBS

    Affinity and Kinetic Characterization of 82672-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 82672-1-RR against Human TIA1 were performed. The affinity constant is 0.385 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82672-1-PBS targets TIA1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TIA1 fusion protein Ag2778 相同性解析による交差性が予測される生物種
Full Name TIA1 cytotoxic granule-associated RNA binding protein
Calculated molecular weight 214 aa, 24 kDa, 43 kDa
Observed molecular weight38-40 kDa
GenBank accession numberBC015944
Gene symbol TIA1
Gene ID (NCBI) 7072
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TIA1, also named as p40-TIA-1, is involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). It possesses nucleolytic activity against cytotoxic lymphocyte target cells. TIA1 may be involved in apoptosis. Two isoforms of this protein exist - 41kDa and 42kDa. one of these was a missense variant (P362L) in TIA1. Similar to the ALS-related disease proteins TDP-43, hnRNPA1, and FUS, TIA1 is an RNA-binding protein containing a prionlike LCD and assembles into membrane-less organelles, including SGs. Postmortem neuropathology of five TIA1 mutations carriers showed a consistent pathological signature with numerous round, hyaline, TAR DNA-binding protein 43 (TDP-43)-positive inclusions.TIA1mutations significantly increased the propensity of TIA1 protein to undergo phase transition. In live cells,TIA1mutations delayed stress granule (SG) disassembly and promoted the accumulation of non-dynamic SGs that harbored TDP-43.