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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using TIAF1 Polyclonal antibody (20328-1-AP)

    WB analysis of PC-3 using 20328-1-AP (same clone as 20328-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20328-1-AP (TIAF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20328-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using TIAF1 Polyclonal antibody (20328-1-AP)

    WB analysis of PC-3 using 20328-1-AP (same clone as 20328-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20328-1-AP (TIAF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20328-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using TIAF1 Polyclonal antibody (20328-1-AP)

    IHC staining of human skin using 20328-1-AP (same clone as 20328-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 20328-1-AP (TIAF1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20328-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using TIAF1 Polyclonal antibody (20328-1-AP)

    IHC staining of human skin using 20328-1-AP (same clone as 20328-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 20328-1-AP (TIAF1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20328-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using TIAF1 Polyclonal antibody (20328-1-AP)

    IF Staining of PC-3 using 20328-1-AP (same clone as 20328-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 20328-1-AP (TIAF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20328-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20328-1-PBS targets TIAF1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TIAF1 fusion protein Ag14023 相同性解析による交差性が予測される生物種
Full Name TGFB1-induced anti-apoptotic factor 1
Calculated molecular weight 115 aa, 12 kDa
Observed molecular weight 12 kDa
GenBank accession numberBC131561
Gene Symbol TIAF1
Gene ID (NCBI) 9220
RRIDAB_10858233
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95411
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.