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  • Featured Product
  • KD/KO Validated

TIAR Polyclonal antibody

TIAR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17649-1-AP

Synonyms

TIAL1, TIA-1-related protein, TIA 1 related protein, TCBP, Nucleolysin TIAR

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TIAR Polyclonal antibody (17649-1-AP)

    WB analysis using 17649-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17649-1-AP (TIAR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using TIAR Polyclonal antibody (17649-1-AP)

    IHC staining of human stomach using 17649-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17649-1-AP (TIAR antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17649-1-AP targets TIAR in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TIAR fusion protein Ag11884 相同性解析による交差性が予測される生物種
Full Name TIA1 cytotoxic granule-associated RNA binding protein-like 1
Calculated molecular weight 42 kDa
Observed molecular weight38-43 kDa
GenBank accession numberBC030025
Gene symbol TIAR
Gene ID (NCBI) 7073
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TIAR, also named as TIA 1 related protein, is an RNA-binding protein which is associated with apoptosis. It has been shown that mouse with disrupted TIAR gene show high levels of embryonic lethality. TIAR plays an important role in cytoplasm and nucleus where it can control translation of some specific mRNAs and activate splicing of exons with weak 5-splice sites.. Besides, TIAR also has the 28 kDa and 50 kDa proteins for TIAR exists in multiple alternative splicing forms.(PMID: 12533540, 7533298, 22851315, 8176212)

Protocols

Product Specific Protocols
WB protocol for TIAR antibody 17649-1-APDownload protocol
IHC protocol for TIAR antibody 17649-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

HuR and TIA1/TIAL1 Are Involved in Regulation of Alternative Splicing of SIRT1 Pre-mRNA.

Authors - Wenhui Zhao
  • KD Validated
View Article