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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TIAM1 Polyclonal antibody

TIAM1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27694-1-AP

Synonyms

TIAM 1, TIAM1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TIAM1 Polyclonal antibody (27694-1-AP)

    WB analysis using 27694-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27694-1-AP (TIAM1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TIAM1 Polyclonal antibody (27694-1-AP)

    WB analysis using 27694-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27694-1-AP (TIAM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HCT 116 cells using TIAM1 Polyclonal antibody (27694-1-AP)

    WB analysis of HCT 116 using 27694-1-AP

    HCT 116 cells were subjected to SDS PAGE followed by western blot with 27694-1-AP (TIAM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TIAM1 Polyclonal antibody (27694-1-AP)

    WB analysis using 27694-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27694-1-AP (TIAM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHEK-293T cells, HCT 116 cells, Neuro-2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

27694-1-AP targets TIAM1 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TIAM1 fusion protein Ag26777 相同性解析による交差性が予測される生物種
Full Name T-cell lymphoma invasion and metastasis 1
Calculated molecular weight 178 kDa
Observed molecular weight178 kDa
GenBank accession numberNM_003253
Gene symbol TIAM1
Gene ID (NCBI) 7074
RRIDAB_2918127
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TIAM1 antibody 27694-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

Phosphorylated SHMT2 Regulates Oncogenesis Through m6 A Modification in Lung Adenocarcinoma

Authors - Tianyu Han
View Article