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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TIAR Monoclonal antibody, PBS Only

TIAR Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E8D12

Cat no : 66907-1-PBS

Synonyms

TIAL1, TIA-1-related protein, TIA 1 related protein, TCBP, Nucleolysin TIAR

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TIAR Monoclonal antibody (66907-1-Ig)

    WB analysis using 66907-1-Ig (same clone as 66907-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66907-1-Ig (TIAR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using TIAR Monoclonal antibody (66907-1-Ig)

    WB analysis of RAW 264.7 using 66907-1-Ig (same clone as 66907-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66907-1-Ig (TIAR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TIAR Monoclonal antibody (66907-1-Ig)

    WB analysis using 66907-1-Ig (same clone as 66907-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66907-1-Ig (TIAR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using TIAR Monoclonal antibody (66907-1-Ig)

    WB analysis of HSC-T6 using 66907-1-Ig (same clone as 66907-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66907-1-Ig (TIAR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TIAR Monoclonal antibody (66907-1-Ig)

    IHC staining of human breast cancer using 66907-1-Ig (same clone as 66907-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66907-1-Ig (TIAR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TIAR Monoclonal antibody (66907-1-Ig)

    IHC staining of human breast cancer using 66907-1-Ig (same clone as 66907-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66907-1-Ig (TIAR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using TIAR Monoclonal antibody (66907-1-Ig)

    IHC staining of human ovary tumor using 66907-1-Ig (same clone as 66907-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66907-1-Ig (TIAR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using TIAR Monoclonal antibody (66907-1-Ig)

    IHC staining of human ovary tumor using 66907-1-Ig (same clone as 66907-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66907-1-Ig (TIAR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TIAR Monoclonal antibody (66907-1-Ig)

    IF Staining of MCF-7 using 66907-1-Ig (same clone as 66907-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66907-1-Ig (TIAR antibody) at dilution of 1:100. This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using TIAR Monoclonal antibody (66907-1-Ig)

    FC experiment of MCF-7 using 66907-1-Ig (same clone as 66907-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human TIAR (66907-1-Ig, Clone:1E8D12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66907-1-PBS targets TIAR in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity rat, mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TIAR fusion protein Ag11981 相同性解析による交差性が予測される生物種
Full Name TIA1 cytotoxic granule-associated RNA binding protein-like 1
Calculated molecular weight 42 kDa
Observed molecular weight 39 and 41 kDa
GenBank accession numberBC030025
Gene symbol TIAR
Gene ID (NCBI) 7073
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TIAR, also named as TIA 1 related protein, is an RNA-binding protein which is associated with apoptosis. It has been shown that mouse with disrupted TIAR gene show high levels of embryonic lethality. TIAR plays an important role in cytoplasm and nucleus where it can control translation of some specific mRNAs and activate splicing of exons with weak 5-splice sites. 66907-1-Ig detects 39 and 41 kDa bands in SDS-PAGE. Besides, TIAR also has the 28 kDa and 50 kDa proteins for TIAR exists in multiple alternative splicing forms.(PMID: 12533540, 7533298, 22851315, 8176212)