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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRIF/TICAM1 Polyclonal antibody

TRIF/TICAM1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23288-1-AP

Synonyms

PRVTIRB, TICAM 1, TICAM1, TRIF, TRIF/TICAM1

Validation Data Gallery

Applications:
  • WB analysis using 23288-1-AP

    WB analysis using 23288-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23288-1-AP (TRIF/TICAM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of Raji using 23288-1-AP

    WB analysis of Raji using 23288-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 23288-1-AP (TRIF/TICAM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of Ramos using 23288-1-AP

    WB analysis of Ramos using 23288-1-AP

    Ramos cells were subjected to SDS PAGE followed by western blot with 23288-1-AP (TRIF/TICAM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 23288-1-AP

    IHC staining of human brain using 23288-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23288-1-AP (TRIF/TICAM1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human brain using 23288-1-AP

    IHC staining of human brain using 23288-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23288-1-AP (TRIF/TICAM1 antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human liver using 23288-1-AP

    IHC staining of human liver using 23288-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23288-1-AP (TRIF/TICAM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 23288-1-AP

    IHC staining of human liver using 23288-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23288-1-AP (TRIF/TICAM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human testis using 23288-1-AP

    IHC staining of human testis using 23288-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23288-1-AP (TRIF/TICAM1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 23288-1-AP

    IHC staining of human testis using 23288-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23288-1-AP (TRIF/TICAM1 antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inMOLT-4 cells, Ramos cells, Raji cells
Positive IHC detected inhuman brain tissue, human testis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

23288-1-AP targets TRIF/TICAM1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, rat, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIF/TICAM1 fusion protein Ag19779 相同性解析による交差性が予測される生物種
Full Name toll-like receptor adaptor molecule 1
Calculated molecular weight 712 aa, 76 kDa
Observed molecular weight 66 kDa, 76 kDa
GenBank accession numberBC035331
Gene symbol TICAM1
Gene ID (NCBI) 148022
RRIDAB_2879247
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TRIF/TICAM1 antibody 23288-1-APDownload protocol
IHC protocol for TRIF/TICAM1 antibody 23288-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Adv Mater

Manganese@Albumin Nanocomplex and Its Assembled Nanowire Activate TLR4-Dependent Signaling Cascades of Macrophages

Authors - Shuodan Huang
View Article
mouseWB

Immunity

The gut microbiota prime systemic antiviral immunity via the cGAS-STING-IFN-I axis.

Authors - Saskia F Erttmann
View Article
humanWB

J Med Virol

SARS-CoV-2 NSP8 suppresses type I and III IFN responses by modulating the RIG-I/MDA5, TRIF, and STING signaling pathways

Authors - Jian Deng
View Article
pigWB

Nucleic Acids Res

The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response.

Authors - Xin Zong
View Article
humanWB

Biochim Biophys Acta Mol Basis Dis

Gut-derived 4-hydroxyphenylacetic acid attenuates sepsis-induced acute kidney injury by upregulating ARC to inhibit necroptosis

Authors - Sheng An
View Article
humanWB

Oncotarget

Monophosphoryl lipid a attenuates radiation injury through TLR4 activation.

Authors - Jiaming Guo
View Article