TRIF/TICAM1 Polyclonal antibody

Name: TRIF/TICAM1 Polyclonal antibody
Brand: Proteintech
SKU: 23288-1-AP

TRIF/TICAM1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 23288-1-AP

Synonyms

TICAM1, TRIF, TICAM 1, Putative NF-kappa-B-activating protein 502H, PRVTIRB

Validation Data Gallery

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AllWBIHC

WB analysis using 23288-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 23288-1-AP (TRIF/TICAM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 23288-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 23288-1-AP (TRIF/TICAM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Ramos using 23288-1-AP
Ramos cells were subjected to SDS PAGE followed by western blot with 23288-1-AP (TRIF/TICAM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human brain using 23288-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23288-1-AP (TRIF/TICAM1 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human brain using 23288-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23288-1-AP (TRIF/TICAM1 antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human testis using 23288-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23288-1-AP (TRIF/TICAM1 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human testis using 23288-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23288-1-AP (TRIF/TICAM1 antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human liver using 23288-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23288-1-AP (TRIF/TICAM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 23288-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23288-1-AP (TRIF/TICAM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 23288-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	MOLT-4 cells, Ramos cells, Raji cells
Positive IHC detected in	human brain tissue, human testis tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 19 publications below
IHC	See 1 publications below
IF	See 2 publications below

Product Information

23288-1-AP targets TRIF/TICAM1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TRIF/TICAM1 fusion protein Ag19779 相同性解析による交差性が予測される生物種
Full Name	toll-like receptor adaptor molecule 1
Calculated molecular weight	712 aa, 76 kDa
Observed molecular weight	66-76 kDa
GenBank accession number	BC035331
Gene symbol	TRIF
Gene ID (NCBI)	148022
RRID	AB_2879247
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TRIF/TICAM1 antibody 23288-1-AP	Download protocol
IHC protocol for TRIF/TICAM1 antibody 23288-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Adv Mater Manganese@Albumin Nanocomplex and Its Assembled Nanowire Activate TLR4-Dependent Signaling Cascades of Macrophages Authors - Shuodan Huang View Article
mouse	WB	Immunity The gut microbiota prime systemic antiviral immunity via the cGAS-STING-IFN-I axis. Authors - Saskia F Erttmann View Article
human	WB	J Med Virol SARS-CoV-2 NSP8 suppresses type I and III IFN responses by modulating the RIG-I/MDA5, TRIF, and STING signaling pathways Authors - Jian Deng View Article
pig	WB	Nucleic Acids Res The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response. Authors - Xin Zong View Article
human	WB	Biochim Biophys Acta Mol Basis Dis Gut-derived 4-hydroxyphenylacetic acid attenuates sepsis-induced acute kidney injury by upregulating ARC to inhibit necroptosis Authors - Sheng An View Article
human	WB	Oncotarget Monophosphoryl lipid a attenuates radiation injury through TLR4 activation. Authors - Jiaming Guo View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRIF/TICAM1 Polyclonal antibody

TRIF/TICAM1 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 23288-1-AP

WB analysis of Raji using 23288-1-AP

WB analysis of Ramos using 23288-1-AP

IHC staining of human brain using 23288-1-AP

IHC staining of human brain using 23288-1-AP

IHC staining of human testis using 23288-1-AP

IHC staining of human testis using 23288-1-AP

IHC staining of human liver using 23288-1-AP

IHC staining of human liver using 23288-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Manganese@Albumin Nanocomplex and Its Assembled Nanowire Activate TLR4-Dependent Signaling Cascades of Macrophages

The gut microbiota prime systemic antiviral immunity via the cGAS-STING-IFN-I axis.

SARS-CoV-2 NSP8 suppresses type I and III IFN responses by modulating the RIG-I/MDA5, TRIF, and STING signaling pathways

The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response.

Gut-derived 4-hydroxyphenylacetic acid attenuates sepsis-induced acute kidney injury by upregulating ARC to inhibit necroptosis

Monophosphoryl lipid a attenuates radiation injury through TLR4 activation.