Validation Data Gallery
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Tested Applications
| Positive WB detected in | K-562 cells, MCF-7 cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
19329-1-AP targets Tie1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Tie1 fusion protein Ag4728 相同性解析による交差性が予測される生物種 |
| Full Name | tyrosine kinase with immunoglobulin-like and EGF-like domains 1 |
| Calculated molecular weight | 1138 aa, 125 kDa |
| Observed molecular weight | 154 kDa |
| GenBank accession number | BC038239 |
| Gene Symbol | TIE1 |
| Gene ID (NCBI) | 7075 |
| RRID | AB_10863655 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35590 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Tie1 antibody 19329-1-AP | Download protocol |
| FC protocol for Tie1 antibody 19329-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Engineered model of heart tissue repair for exploring fibrotic processes and therapeutic interventions | ||