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TIFA Polyclonal antibody
TIFA Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 11333-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, L02 cells, TT cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
11333-1-AP targets TIFA in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TIFA fusion protein Ag1876 相同性解析による交差性が予測される生物種 |
| Full Name | TRAF-interacting protein with forkhead-associated domain |
| Calculated molecular weight | 21 kDa |
| Observed molecular weight | 21 kDa |
| GenBank accession number | BC014259 |
| Gene symbol | TIFA |
| Gene ID (NCBI) | 92610 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TIFA (TRAF-interacting protein with FHA domain-containing protein A ), also called T2BP, can interact with TRAF6 and plays an important role in innate immunity (PMID: 32910997; 35635239). Oligomerization of TIFA, which induces oligomerization and polyubiquitinylation of TRAF6, in turn activates TAK and IKK, finally mediates NF‐κB activation in the Toll‐like receptor 4/interleukin‐1 signaling pathway (PMID: 15492226; 22566686). It has been reported that TIFA also plays various roles in different cancer types (PMID: 26501855; 29263897).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TIFA antibody 11333-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
