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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TIGIT Recombinant antibody, PBS Only (Capture)

TIGIT Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240423D3

Cat no : 83545-1-PBS

Synonyms

TIGIT, V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain-containing protein 3, VSIG9, VSTM3, 240423D3, T-cell immunoreceptor with Ig and ITIM domains

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TIGIT Recombinant antibody (83545-1-RR)

    WB analysis using 83545-1-RR (same clone as 83545-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83545-1-RR (TIGIT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83545-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using TIGIT Recombinant antibody (83545-1-RR)

    IF Staining of Jurkat using 83545-1-RR (same clone as 83545-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using TIGIT antibody (83545-1-RR, Clone: 240423D3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83545-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00528-2

    Cytometric bead array standard curve of MP00528-2

    Cytometric bead array standard curve of MP00528-2, TIGIT Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83545-1-PBS. Detection antibody: 83545-4-PBS. Standard: Eg0749. Range: 0.625-40 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83545-1-PBS targets TIGIT as part of a matched antibody pair:

MP00528-2: 83545-1-PBS capture and 83545-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name T cell immunoreceptor with Ig and ITIM domains
Calculated molecular weight26 kDa
GenBank accession numberNM_173799.4
Gene symbol TIGIT
Gene ID (NCBI) 201633
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.