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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TIGIT Recombinant antibody (83545-1-RR)

    WB analysis using 83545-1-RR (same clone as 83545-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83545-1-RR (TIGIT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83545-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00528-2

    Sandwich ELISA standard curve of MP00528-2

    Sandwich ELISA standard curve of MP00528-2, Human TIGIT Recombinant Matched Antibody Pair - PBS only. 83545-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0749. 83545-4-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

  • Sample test of MP00528-2

    Sample test of MP00528-2

    The mean TIGIT concentration was determined to be 1,338.3 pg/mL in NK-92 cell extract based on a 2.0 mg/mL extract load.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TIGIT Recombinant antibody (83545-1-RR)

    IHC staining of human tonsillitis using 83545-1-RR (same clone as 83545-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83545-1-RR (TIGIT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83545-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using TIGIT Recombinant antibody (83545-1-RR)

    IF Staining of mouse spleen using 83545-1-RR (same clone as 83545-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using TIGIT antibody (83545-1-RR, Clone: 240423D3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83545-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using TIGIT Recombinant antibody (83545-1-RR)

    IF Staining of Jurkat using 83545-1-RR (same clone as 83545-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using TIGIT antibody (83545-1-RR, Clone: 240423D3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83545-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00528-2

    Cytometric bead array standard curve of MP00528-2

    Cytometric bead array standard curve of MP00528-2, TIGIT Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83545-1-PBS. Detection antibody: 83545-4-PBS. Standard: Eg0749. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 83545-1-RR

    Affinity and Kinetic Characterization of 83545-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83545-1-RR against Human TIGIT were performed. The affinity constant is below 1 pM.

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TIGIT Recombinant antibody, PBS Only (Capture)

TIGIT Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83545-1-PBS
Clone No.240423D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

VSTM3, VSIG9, V-set and transmembrane domain-containing protein 3, V-set and immunoglobulin domain-containing protein 9, T-cell immunoreceptor with Ig and ITIM domains

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83545-1-PBS targets TIGIT as part of a matched antibody pair:

MP00528-2: 83545-1-PBS capture and 83545-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name T cell immunoreceptor with Ig and ITIM domains
Calculated molecular weight26 kDa
Observed molecular weight44 kDa
GenBank accession numberNM_173799.4
Gene Symbol TIGIT
Gene ID (NCBI) 201633
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ495A1-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).