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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human TIGIT Rabbit Recombinant Antibody, PBS Only

TIGIT Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240423C12

Cat no : 98023-1-PBS

Synonyms

VSTM3, VSIG9, V-set and transmembrane domain-containing protein 3, V-set and immunoglobulin domain-containing protein 9, T-cell immunoreceptor with Ig and ITIM domains

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human TIGIT Rabbit Recombinant Antibody (98023-1-RR)

    FC experiment of human PBMCs using 98023-1-RR (same clone as 98023-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TIGIT Rabbit Recombinant Antibody (98023-1-RR, Clone: 240423C12) or Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with APC Anti-Human CD45RO. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98023-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human TIGIT Rabbit Recombinant Antibody (98023-1-RR)

    FC experiment of human PBMCs using 98023-1-RR (same clone as 98023-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TIGIT Rabbit Recombinant Antibody (98023-1-RR, Clone: 240423C12) (red) or Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98023-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98023-1-RR

    Affinity and Kinetic Characterization of 98023-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98023-1-RR against Human TIGIT were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 98023-1-PBS is TIGIT Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name T cell immunoreceptor with Ig and ITIM domains
Calculated molecular weight26 kDa
GenBank accession numberNM_173799.4
Gene symbol TIGIT
Gene ID (NCBI) 201633
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).