Validation Data Gallery
Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
PE-65079 targets TIGIT in FC applications and shows reactivity with Mouse samples.
| Tested Reactivity | Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | N/A 相同性解析による交差性が予測される生物種 |
| Full Name | T cell immunoreceptor with Ig and ITIM domains |
| GenBank accession number | NM_001146325 |
| Gene Symbol | Tigit |
| Gene ID (NCBI) | 100043314 |
| RRID | AB_2883875 |
| Conjugate | PE Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 496 nm, 565 nm / 578 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P86176 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.1% gelatin , pH 7.2 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PE TIGIT antibody PE-65079 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |