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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TIM3 Recombinant antibody

TIM3 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

5A8

Cat no : 82836-1-RR

Synonyms

T-cell immunoglobulin mucin receptor 3, T-cell immunoglobulin and mucin domain-containing protein 3, HAVcr-2, HAVCR2, CD366

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TIM3 Recombinant antibody (82836-1-RR)

    WB analysis using 82836-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82836-1-RR (TIM3 antibody) at dilution of 1:3500 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82836-1-RR

    Affinity and Kinetic Characterization of 82836-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82836-1-RR against Human TIM3 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inPC-3 cells, THP-1 cells, Daudi cells, RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:7000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82836-1-RR targets TIM3 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TIM3 fusion protein Ag2459 相同性解析による交差性が予測される生物種
Full Name hepatitis A virus cellular receptor 2
Calculated molecular weight 301 aa, 33 kDa
Observed molecular weight70 kDa
GenBank accession numberBC020843
Gene symbol TIM3
Gene ID (NCBI) 84868
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).

Protocols

Product Specific Protocols
WB protocol for TIM3 antibody 82836-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols