TIM3 Recombinant antibody
TIM3 Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
5A8
Cat no : 82836-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | PC-3 cells, THP-1 cells, Daudi cells, RAW 264.7 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:7000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
82836-1-RR targets TIM3 in WB, ELISA applications and shows reactivity with Human, Mouse samples.
Tested Reactivity | Human, Mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | TIM3 fusion protein Ag2459 相同性解析による交差性が予測される生物種 |
Full Name | hepatitis A virus cellular receptor 2 |
Calculated molecular weight | 301 aa, 33 kDa |
Observed molecular weight | 70 kDa |
GenBank accession number | BC020843 |
Gene symbol | TIM3 |
Gene ID (NCBI) | 84868 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).
Protocols
Product Specific Protocols | |
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WB protocol for TIM3 antibody 82836-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |