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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TIM3 Monoclonal antibody, PBS Only

TIM3 Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse, rat

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

4C4G3

Cat no : 60355-1-PBS

Synonyms

CD366, HAVcr 2, HAVCR2, KIM 3, T cell membrane protein 3, Tim 3, TIM3, TIM-3, TIMD 3, TIMD3

Validation Data Gallery

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  • Western Blot (WB) analysis of Daudi cells using TIM3 Monoclonal antibody (60355-1-Ig)

    WB analysis of Daudi using 60355-1-Ig (same clone as 60355-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60355-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig thymus tissue using TIM3 Monoclonal antibody (60355-1-Ig)

    WB analysis of pig thymus using 60355-1-Ig (same clone as 60355-1-PBS)

    pig thymus tissue were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60355-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using TIM3 Monoclonal antibody (60355-1-Ig)

    WB analysis of rat heart using 60355-1-Ig (same clone as 60355-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60355-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ROS1728 cells using TIM3 Monoclonal antibody (60355-1-Ig)

    WB analysis of ROS1728 using 60355-1-Ig (same clone as 60355-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60355-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using TIM3 Monoclonal antibody (60355-1-Ig)

    WB analysis of human heart using 60355-1-Ig (same clone as 60355-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60355-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using TIM3 Monoclonal antibody (60355-1-Ig)

    WB analysis of RAW 264.7 using 60355-1-Ig (same clone as 60355-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60355-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using TIM3 Monoclonal antibody (60355-1-Ig)

    WB analysis of PC-3 using 60355-1-Ig (same clone as 60355-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60355-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Daudi cells using TIM3 Monoclonal antibody (60355-1-Ig)

    FC experiment of Daudi using 60355-1-Ig (same clone as 60355-1-PBS)

    1X10^6 Daudi cells were stained with 0.20 ug Anti-Human TIM3 (60355-1-Ig, Clone: 4C4G3) (red) or 0.20 ug isotype control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Cells were fixed with 90% MeOH. This data was developed using the same antibody clone with 60355-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60355-1-PBS targets TIM3 in WB, FC, ELISA applications and shows reactivity with human, pig, mouse, rat samples.

Tested Reactivity human, pig, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TIM3 fusion protein Ag16901 相同性解析による交差性が予測される生物種
Full Name hepatitis A virus cellular receptor 2
Calculated molecular weight 301 aa, 33 kDa
Observed molecular weight 33 kDa, 50-70 kDa
GenBank accession numberBC020843
Gene symbol HAVCR2
Gene ID (NCBI) 84868
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa detected by this monoclonal antibody probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).