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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human TIM3 Rabbit Recombinant Antibody, PBS Only

TIM3 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241422H1

Cat no : 98115-1-PBS

Synonyms

T-cell immunoglobulin mucin receptor 3, T-cell immunoglobulin and mucin domain-containing protein 3, HAVcr-2, HAVCR2, CD366

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human TIM3 Rabbit Recombinant Antibody (98115-1-RR)

    FC experiment of human PBMCs using 98115-1-RR (same clone as 98115-1-PBS)

    1x10^6 untreated or anti-CD3/CD28 treated human PBMCs were surface stained with 0.25 ug Anti-Human TIM3 Rabbit Recombinant Antibody (98115-1-RR, Clone: 241422H1) or 0.25 ug Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD4 (RPA-T4) (APC-65143, Clone: RPA-T4). Cells were not fixed. This data was developed using the same antibody clone with 98115-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human TIM3 Rabbit Recombinant Antibody (98115-1-RR)

    FC experiment of human PBMCs using 98115-1-RR (same clone as 98115-1-PBS)

    1x10^6 anti-CD3/CD28 treated human PBMCs were surface stained with 0.25 ug Anti-Human TIM3 Rabbit Recombinant Antibody (98115-1-RR, Clone: 241422H1) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human TIM3 Rabbit Recombinant Antibody (98115-1-RR, Clone: 241422H1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed. This data was developed using the same antibody clone with 98115-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98115-1-RR

    Affinity and Kinetic Characterization of 98115-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98115-1-RR against Human TIM3 were performed. The affinity constant is 0.688 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98115-1-PBS targets TIM3 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name hepatitis A virus cellular receptor 2
Calculated molecular weight 301 aa, 33 kDa
GenBank accession numberBC020843
Gene symbol TIM3
Gene ID (NCBI) 84868
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920).