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Species Reactivity

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ChromoTek TIM3 VHH, recombinant binding protein for 2x Cys conjugation

Alpaca anti-TIM3 VHH, purified recombinant binding protein. Suitable for for cysteine conjugation with thiol-reactive reagents, e.g. maleimides. Note: unconjugated VHHs are not suited for usage without prior labeling, since they contain reactive Cysteines. Shipment and storage buffers contain TCEP to keep Cysteins reduced.

Host/Type

Alpaca VHH

Reactivity

Human

Applications

Conjugation

Conjugate

Unconjugated

Cat no : tmtCys2

Synonyms

TIM3-Nanobody, TIM3-single domain antibody (sdAb), CD366, HAVcr 2, HAVCR2, KIM 3, T cell membrane protein 3, Tim 3, TIM3, TIM-3, TIMD 3, TIMD3

Validation Data Gallery

  • The affinity of anti-human TIM3 recombinant VHH towards human TIM3 was determined using biolayer interferometry (BLI). Biotinylated, recombinant human TIM3 was immobilized on Streptavidin biosensors and assayed with 3 to 50 nM of CoraLite® Plus 647-conjugated TIM3 VHH (CL647-tmt). Fit indicates a 1:1 binding model fitted to the data.

    The affinity of anti-human TIM3 recombinant VHH towards human TIM3 was determined using biolayer interferometry (BLI). Biotinylated, recombinant human TIM3 was immobilized on Streptavidin biosensors and assayed with 3 to 50 nM of CoraLite® Plus 647-conjugated TIM3 VHH (CL647-tmt). Fit indicates a 1:1 binding model fitted to the data.

Product Information

Alpaca anti-TIM3 VHH, purified recombinant binding protein. Suitable for for cysteine conjugation with thiol-reactive reagents, e.g. maleimides. Note: unconjugated VHHs are not suited for usage without prior labeling, since they contain reactive Cysteines. Shipment and storage buffers contain TCEP to keep Cysteins reduced.

ApplicationsConjugation
ReactivityHuman
ConjugateUnconjugated
Type Nanobody
ClassRecombinant
HostAlpaca
Affinityin the picomolar range, below the assay limit (biolayer interferometry)
Molecular Weight14.824 kDa
FormLiquid
RRIDAB_3101920
Storage Buffer10 mM HEPES, 500 mM NaCl, pH 7.0, 1 mM TCEP, 0.09% sodium azide
Storage ConditionStore at -20°C; Avoid exposure to light. Shipped at dry ice.
BackgroundTIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa detected by this monoclonal antibody probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).