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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TIMP2 Polyclonal antibody

TIMP2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 26647-1-AP

Synonyms

CSC 21K, Metalloproteinase inhibitor 2, TIMP 2, TIMP2

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse lung tissue using TIMP2 Polyclonal antibody (26647-1-AP)

    IHC staining of mouse lung using 26647-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26647-1-AP (TIMP2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using TIMP2 Polyclonal antibody (26647-1-AP)

    IHC staining of mouse lung using 26647-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26647-1-AP (TIMP2 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TIMP2 Polyclonal antibody (26647-1-AP)

    IHC staining of human kidney using 26647-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26647-1-AP (TIMP2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TIMP2 Polyclonal antibody (26647-1-AP)

    IHC staining of human kidney using 26647-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26647-1-AP (TIMP2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive IHC detected inmouse lung tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26647-1-AP is TIMP2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TIMP2 fusion protein Ag24436 相同性解析による交差性が予測される生物種
Full Name TIMP metallopeptidase inhibitor 2
Calculated molecular weight 220 aa, 24 kDa
GenBank accession numberBC052605
Gene symbol TIMP2
Gene ID (NCBI) 7077
RRIDAB_2880587
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The TIMPs are natural highly specific endogenous inhibitors of MMPs. Human TIMP family consists of four 21-28 kDa proteins known as TIMP1, TIMP2, TIMP3, and TIMP4 encoded by four paralogous genes.TIMPs are vital to the maintenance of ECM homeostasis primarily through their MMP inhibitory functions. TIMP2, a member of the TIMP family, regulates the proteolytic activity of all MMPs and is involved in cell differentiation, growth, migration, angiogenesis, and apoptosis. Urinary TIMP2 has been recently recognized as an early biomarker to predict Acute kidney injury (AKI) in critically ill patients.

Protocols

Product Specific Protocols
IHC protocol for TIMP2 antibody 26647-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols