Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse spleen tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| ChIP | See 2 publications below |
Product Information
11368-1-AP targets TIN2 in WB, IF/ICC, chip, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TIN2 fusion protein Ag1922 相同性解析による交差性が予測される生物種 |
| Full Name | TERF1 (TRF1)-interacting nuclear factor 2 |
| Calculated molecular weight | 451 aa, 50 kDa |
| Observed molecular weight | 50 kDa, 40 kDa |
| GenBank accession number | BC005030 |
| Gene Symbol | TINF2 |
| Gene ID (NCBI) | 26277 |
| RRID | AB_2205100 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BSI4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TIN2, also named as TINF2, is a component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. TIN2 has some isoforms with MW 50 kDa (TIN2L), 40 kDa (TIN2S) and 13 kDa. This antibody detects TIN2L, TIN2S and the shelterin complex.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TIN2 antibody 11368-1-AP | Download protocol |
| IF protocol for TIN2 antibody 11368-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncotarget BRCA1/2 mutations perturb telomere biology: characterization of structural and functional abnormalities in vitro and in vivo. | ||
Tissue Cell IGF2BPs-regulated TIN2 confers the malignant biological behaviors of gastric cancer cells | ||
Nat Commun Transcription stress at telomeres leads to cytosolic DNA release and paracrine senescence |