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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TINAG-Specific Polyclonal antibody

TINAG-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19747-1-AP

Synonyms

TIN AG, TINAG

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse small intestine tissue using TINAG-Specific Polyclonal antibody (19747-1-AP)

    WB analysis of mouse small intestine using 19747-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 19747-1-AP (TINAG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using TINAG-Specific Polyclonal antibody (19747-1-AP)

    WB analysis of mouse small intestine using 19747-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 19747-1-AP (TINAG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using TINAG-Specific Polyclonal antibody (19747-1-AP)

    WB analysis of human kidney using 19747-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 19747-1-AP (TINAG-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using TINAG-Specific Polyclonal antibody (19747-1-AP)

    WB analysis of human placenta using 19747-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 19747-1-AP (TINAG-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using TINAG-Specific Polyclonal antibody (19747-1-AP)

    IHC staining of human colon using 19747-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 19747-1-AP (TINAG-Specific antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using TINAG-Specific Polyclonal antibody (19747-1-AP)

    IHC staining of human colon using 19747-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 19747-1-AP (TINAG-Specific antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inmouse small intestine tissue, human kidney tissue, human placenta tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

19747-1-AP targets TINAG-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name tubulointerstitial nephritis antigen
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberNM_014464
Gene symbol TINAG
Gene ID (NCBI) 27283
RRIDAB_10642433
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TINAG belongs to the peptidase C1 family. TINAG mediates adhesion of proximal tubule epithelial cells via integrins alpha3-beta1 and alphaV-beta3. It is a non catalytic peptidase C1 family protein. Antibodies against TINAG are found in sera of patients with tubulointerstitial nephritis, a rare autoimmune disorder that causes acute and chronic renal injury.It has 2 isoforms produced by alternative splicing. The antibody is specific to TINAG.

Protocols

Product Specific Protocols
WB protocol for TINAG-Specific antibody 19747-1-APDownload protocol
IHC protocol for TINAG-Specific antibody 19747-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Transl Cancer Res

Modeling of new markers for the diagnosis and prognosis of pancreatic cancer based on the transition from inflammation to cancer

Authors - Yuan Zhou
View Article