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- KD/KO Validated
TINAGL1 Polyclonal antibody
TINAGL1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF-P, IP, ELISA
Conjugate
Unconjugated
Cat no : 12077-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human placenta tissue, human adrenal gland tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:300-1:1500 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 7 publications below |
| IHC | See 2 publications below |
| IP | See 1 publications below |
Product Information
12077-1-AP targets TINAGL1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TINAGL1 fusion protein Ag2721 相同性解析による交差性が予測される生物種 |
| Full Name | tubulointerstitial nephritis antigen-like 1 |
| Calculated molecular weight | 467 aa, 52 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC009048 |
| Gene symbol | TINAGL1 |
| Gene ID (NCBI) | 64129 |
| RRID | AB_2058942 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Tubulointerstitial nephritis antigen-like 1 (Tinagl1), a secreted extracellular protein, was initially identified as a putative component of the ECM. Tinagl1 is most commonly associated with embryonic development, including the heart and adrenal glands. Low levels of Tinagl1 expression can be dire for craniofacial development and for female fertility.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TINAGL1 antibody 12077-1-AP | Download protocol |
| IHC protocol for TINAGL1 antibody 12077-1-AP | Download protocol |
| IF protocol for TINAGL1 antibody 12077-1-AP | Download protocol |
| IP protocol for TINAGL1 antibody 12077-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochim Biophys Acta Mol Cell Res Functional analysis reveals that Tinagl1 is required for normal muscle development in mice through the activation of ERK signaling.
| ||
J Transl Med Tubulointerstitial nephritis antigen-like 1 from cancer-associated fibroblasts contribute to the progression of diffuse-type gastric cancers through the interaction with integrin β1 | ||
Cell Signal YTHDF1 facilitates esophageal cancer progression via augmenting m6A-dependent TINAGL1 translation
| ||
Matrix Biol Plus Matrisome proteomics reveals novel mediators of muscle remodeling with aerobic exercise training | ||
Cancer Cell Tinagl1 Suppresses Triple-Negative Breast Cancer Progression and Metastasis by Simultaneously Inhibiting Integrin/FAK and EGFR Signaling. |
