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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TINP1 Monoclonal antibody, PBS Only

TINP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E3B11

Cat no : 66374-1-PBS

Synonyms

CDK105, Hairy cell leukemia protein 1, HCL G1, HUSSY29, NSA2, TINP1, YR 29

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using TINP1 Monoclonal antibody (66374-1-Ig)

    WB analysis of L02 using 66374-1-Ig (same clone as 66374-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 66374-1-Ig (TINP1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using TINP1 Monoclonal antibody (66374-1-Ig)

    WB analysis of HEK-293 using 66374-1-Ig (same clone as 66374-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66374-1-Ig (TINP1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using TINP1 Monoclonal antibody (66374-1-Ig)

    WB analysis of HepG2 using 66374-1-Ig (same clone as 66374-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66374-1-Ig (TINP1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TINP1 Monoclonal antibody (66374-1-Ig)

    IHC staining of human breast cancer using 66374-1-Ig (same clone as 66374-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66374-1-Ig (TINP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TINP1 Monoclonal antibody (66374-1-Ig)

    IHC staining of human breast cancer using 66374-1-Ig (same clone as 66374-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66374-1-Ig (TINP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using TINP1 Monoclonal antibody (66374-1-Ig)

    IF Staining of HEK-293 using 66374-1-Ig (same clone as 66374-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TINP1 antibody (66374-1-Ig, Clone: 1E3B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using TINP1 Monoclonal antibody (66374-1-Ig)

    IF Staining of HEK-293 using 66374-1-Ig (same clone as 66374-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TINP1 antibody (66374-1-Ig, Clone: 1E3B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66374-1-PBS targets TINP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TINP1 fusion protein Ag8822 相同性解析による交差性が予測される生物種
Full Name TGF beta-inducible nuclear protein 1
Calculated molecular weight 260 aa, 30 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC005288
Gene symbol TINP1
Gene ID (NCBI) 10412
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TINP1 (TGF beta inducible nuclear protein 1) was widely expressed in various cell lines. TINP1 overexpression significantly promoted tumor cell proliferation, which may be associated with the downregulation of p53 expression. Furthermore, TINP1 promoted a number of cell lines to the S phase of the cell cycle. TINP1 promotes cell proliferation and significantly reduces p53 and p21 expression.