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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TIRAP Polyclonal antibody

TIRAP Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26563-1-AP

Synonyms

TIR domain-containing adapter protein, MyD88-2, MyD88 adapter-like protein, MyD88 adapter like protein, MAL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TIRAP Polyclonal antibody (26563-1-AP)

    WB analysis using 26563-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26563-1-AP (TIRAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHT-29 cells, HeLa cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26563-1-AP targets TIRAP in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TIRAP fusion protein Ag24230 相同性解析による交差性が予測される生物種
Full Name toll-interleukin 1 receptor (TIR) domain containing adaptor protein
Calculated molecular weight 256 aa, 28 kDa
Observed molecular weight26-28 kDa
GenBank accession numberBC032474
Gene symbol TIRAP
Gene ID (NCBI) 114609
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TIRAP, also known as MyD88-adaptor Like (MAL), is a crucial intracellular signaling molecule that plays a significant role in the regulation of immune responses, particularly in the context of Toll-like receptor (TLR)-mediated innate immune signaling. It functions as an adapter molecule that associates with receptor-mediated activation of host immune signaling. The innate immune system recognizes microbial pathogens through receptors, including TLRs, which identify pathogen-associated molecular patterns (PAMPs).

Protocols

Product Specific Protocols
WB protocol for TIRAP antibody 26563-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols