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- KD/KO Validated
TK1 Polyclonal antibody
TK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 15691-1-AP
Synonyms
Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HEK-293 cells, MCF-7 cells, SW 1990 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 9 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
15691-1-AP targets TK1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TK1 fusion protein Ag8249 相同性解析による交差性が予測される生物種 |
| Full Name | thymidine kinase 1, soluble |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 25-30 kDa |
| GenBank accession number | BC007986 |
| Gene symbol | TK1 |
| Gene ID (NCBI) | 7083 |
| RRID | AB_2203613 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TK1(Thymidine kinase, cytosolic) belongs to the thymidine kinase family and is a regulatory enzyme that can interchange between its dimeric and tetrameric forms, which have low and high catalytic efficiencies, respectively. TK1 is involved in DNA synthesis and an important proliferation marker(PMID:21178264).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TK1 antibody 15691-1-AP | Download protocol |
| IHC protocol for TK1 antibody 15691-1-AP | Download protocol |
| IF protocol for TK1 antibody 15691-1-AP | Download protocol |
| IP protocol for TK1 antibody 15691-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Thymidine kinase 1 drives hepatocellular carcinoma in enzyme-dependent and -independent manners
| ||
Theranostics Intravenous transplantation of olfactory ensheathing cells reduces neuroinflammation after spinal cord injury via interleukin-1 receptor antagonist. | ||
Oxid Med Cell Longev lncRNA IGF2-AS Regulates Nucleotide Metabolism by Mediating HMGA1 to Promote Pyroptosis of Endothelial Progenitor Cells in Sepsis Patients. | ||
Cancer Biol Ther rAAV9-UPII-TK-EGFP can precisely transduce a suicide gene and inhibit the growth of bladder tumors. | ||
Adv Biol Regul Fhit and Wwox loss-associated genome instability: A genome caretaker one-two punch. | ||
J Cell Mol Med Down-regulation of TFAM increases the sensitivity of tumour cells to radiation via p53/TIGAR signalling pathway. |
