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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TK1 Monoclonal antibody

TK1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B1C2

Cat no : 67787-1-Ig

Synonyms

thymidine kinase 1, soluble, Thymidine kinase, cytosolic, TK1, TK2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TK1 Monoclonal antibody (67787-1-Ig)

    WB analysis using 67787-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67787-1-Ig (TK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of various lysates using TK1 Monoclonal antibody (67787-1-Ig)

    WB analysis using 67787-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67787-1-Ig (TK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using TK1 Monoclonal antibody (67787-1-Ig)

    WB analysis using 67787-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67787-1-Ig (TK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TK1 Monoclonal antibody (67787-1-Ig)

    IHC staining of human colon cancer using 67787-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67787-1-Ig (TK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TK1 Monoclonal antibody (67787-1-Ig)

    IHC staining of human colon cancer using 67787-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67787-1-Ig (TK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HCT 116 cells using TK1 Monoclonal antibody (67787-1-Ig)

    IF Staining of HCT 116 using 67787-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using TK1 antibody (67787-1-Ig, Clone: 1B1C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, THP-1 cells, Raji cells, HEK-293 cells, HepG2 cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHCT 116 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67787-1-Ig targets TK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TK1 fusion protein Ag8265 相同性解析による交差性が予測される生物種
Full Name thymidine kinase 1, soluble
Calculated molecular weight 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC007986
Gene symbol TK1
Gene ID (NCBI) 7083
RRIDAB_2918551
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TK1(Thymidine kinase, cytosolic) belongs to the thymidine kinase family and is a regulatory enzyme that can interchange between its dimeric and tetrameric forms, which have low and high catalytic efficiencies, respectively. TK1 is involved in DNA synthesis and an important proliferation marker(PMID:21178264).

Protocols

Product Specific Protocols
WB protocol for TK1 antibody 67787-1-IgDownload protocol
IHC protocol for TK1 antibody 67787-1-IgDownload protocol
IF protocol for TK1 antibody 67787-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Cell Death Dis

m5C-dependent cross-regulation between nuclear reader ALYREF and writer NSUN2 promotes urothelial bladder cancer malignancy through facilitating RABL6/TK1 mRNAs splicing and stabilization

Authors - Ning Wang
View Article