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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TKTL2 Polyclonal antibody (17135-1-AP)

    WB analysis using 17135-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17135-1-AP (TKTL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat testis tissue using TKTL2 Polyclonal antibody (17135-1-AP)

    IHC staining of rat testis using 17135-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17135-1-AP (TKTL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using TKTL2 Polyclonal antibody (17135-1-AP)

    IHC staining of rat testis using 17135-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17135-1-AP (TKTL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TKTL2 Polyclonal antibody (17135-1-AP)

    IF Staining of HepG2 using 17135-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TKTL2 antibody (17135-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

TKTL2 Polyclonal antibody

TKTL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17135-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:2.2.1.1, transketolase like 2, Transketolase like protein 2, Transketolase-like protein 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, mouse testis tissue
Positive IHC detected inrat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17135-1-AP targets TKTL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TKTL2 fusion protein Ag10884 相同性解析による交差性が予測される生物種
Full Name transketolase-like 2
Calculated molecular weight 626 aa, 68 kDa
Observed molecular weight68 kDa
GenBank accession numberBC028707
Gene Symbol TKTL2
Gene ID (NCBI) 84076
RRIDAB_3085516
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0I9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transketolase-like 2 (TKTL2) plays an essential role in total transketolase activity and cell proliferation in cancer cells. TKTL2 is predicted to enable thiamine pyrophosphate binding activity and transketolase activity.

Protocols

Product Specific Protocols
WB protocol for TKTL2 antibody 17135-1-APDownload protocol
IHC protocol for TKTL2 antibody 17135-1-APDownload protocol
IF protocol for TKTL2 antibody 17135-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols