Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22093-1-AP targets TLE1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TLE1 fusion protein Ag17299 相同性解析による交差性が予測される生物種 |
| Full Name | transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) |
| Calculated molecular weight | 770 aa, 83 kDa |
| Observed molecular weight | 85-95 kDa |
| GenBank accession number | BC010100 |
| Gene Symbol | TLE1 |
| Gene ID (NCBI) | 7088 |
| RRID | AB_3669386 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q04724 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TLE1 (Transducin-like enhancer protein 1) is a transcriptional corepressor and binds to a number of transcription factors. It can inhibit NF-kappa-B-regulated gene expression and the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (PMID: 10660609). TLE1 is highly expressed in postnatal brain (PMID: 29758293; 27852056).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TLE1 antibody 22093-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |