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  • Featured Product
  • KD/KO Validated

TLE3 Polyclonal antibody

TLE3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 22094-1-AP

Synonyms

HsT18976, GRG3, GRG 3, ESG3, ESG

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using TLE3 Polyclonal antibody (22094-1-AP)

    WB analysis of HEK-293 using 22094-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22094-1-AP (TLE3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TLE3 Polyclonal antibody (22094-1-AP)

    WB analysis of HEK-293 using 22094-1-AP

    WB result of TLE3 antibody (22094-1-AP, 1:4000) with si-Control and si-TLE3 transfected HEK293 cells.

  • Western Blot (WB) analysis of A431 cells using TLE3 Polyclonal antibody (22094-1-AP)

    WB analysis of A431 using 22094-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 22094-1-AP (TLE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TLE3 Polyclonal antibody (22094-1-AP)

    WB analysis of HepG2 using 22094-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 22094-1-AP (TLE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TLE3 Polyclonal antibody (22094-1-AP)

    WB analysis of HEK-293 using 22094-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22094-1-AP (TLE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using TLE3 Polyclonal antibody (22094-1-AP)

    IP experiment of HepG2 using 22094-1-AP

    IP result of anti-TLE3 (IP:22094-1-AP, 4ug; Detection:22094-1-AP 1:1000) with HepG2 cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TLE3 Polyclonal antibody (22094-1-AP)

    IHC staining of human lung cancer using 22094-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22094-1-AP (TLE3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TLE3 Polyclonal antibody (22094-1-AP)

    IHC staining of human breast cancer using 22094-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22094-1-AP (TLE3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TLE3 Polyclonal antibody (22094-1-AP)

    IHC staining of human breast cancer using 22094-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22094-1-AP (TLE3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TLE3 Polyclonal antibody (22094-1-AP)

    IHC staining of human lung cancer using 22094-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22094-1-AP (TLE3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TLE3 Polyclonal antibody (22094-1-AP)

    IF Staining of HepG2 using 22094-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TLE3 antibody (22094-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22094-1-AP targets TLE3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TLE3 fusion protein Ag17300 相同性解析による交差性が予測される生物種
Full Name transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
Calculated molecular weight 772 aa, 83 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC015729
Gene symbol TLE3
Gene ID (NCBI) 7090
RRIDAB_2878993
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transducin-like enhancer of split 3(TLE3) is a corepressor that binds to a variant of transciption factor, which mediated by CTNNB1 and TCF family members in Wnt signaling. It's suggested that effect of full-length TLE family members be regulated by association with dominant-negative AES.

Protocols

Product Specific Protocols
WB protocol for TLE3 antibody 22094-1-APDownload protocol
IHC protocol for TLE3 antibody 22094-1-APDownload protocol
IF protocol for TLE3 antibody 22094-1-APDownload protocol
IP protocol for TLE3 antibody 22094-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Cells

Differential Regulation of TLE3 in Sertoli Cells of the Testes during Postnatal Development.

Authors - Sangho Lee
  • KD Validated
View Article