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  • Featured Product
  • KD/KO Validated

TLE3 Polyclonal antibody, PBS Only

TLE3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22094-1-PBS

Synonyms

HsT18976, GRG3, GRG 3, ESG3, ESG

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using TLE3 Polyclonal antibody (22094-1-AP)

    WB analysis of HEK-293 using 22094-1-AP (same clone as 22094-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22094-1-AP (TLE3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22094-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using TLE3 Polyclonal antibody (22094-1-AP)

    WB analysis of HEK-293 using 22094-1-AP (same clone as 22094-1-PBS)

    WB result of TLE3 antibody (22094-1-AP, 1:4000) with si-Control and si-TLE3 transfected HEK293 cells. This data was developed using the same antibody clone with 22094-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using TLE3 Polyclonal antibody (22094-1-AP)

    WB analysis of A431 using 22094-1-AP (same clone as 22094-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 22094-1-AP (TLE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22094-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using TLE3 Polyclonal antibody (22094-1-AP)

    WB analysis of HepG2 using 22094-1-AP (same clone as 22094-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 22094-1-AP (TLE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22094-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using TLE3 Polyclonal antibody (22094-1-AP)

    WB analysis of HEK-293 using 22094-1-AP (same clone as 22094-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22094-1-AP (TLE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22094-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using TLE3 Polyclonal antibody (22094-1-AP)

    IP experiment of HepG2 using 22094-1-AP (same clone as 22094-1-PBS)

    IP result of anti-TLE3 (IP:22094-1-AP, 4ug; Detection:22094-1-AP 1:1000) with HepG2 cells lysate 2400ug. This data was developed using the same antibody clone with 22094-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TLE3 Polyclonal antibody (22094-1-AP)

    IHC staining of human lung cancer using 22094-1-AP (same clone as 22094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22094-1-AP (TLE3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22094-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TLE3 Polyclonal antibody (22094-1-AP)

    IHC staining of human breast cancer using 22094-1-AP (same clone as 22094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22094-1-AP (TLE3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22094-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TLE3 Polyclonal antibody (22094-1-AP)

    IHC staining of human breast cancer using 22094-1-AP (same clone as 22094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22094-1-AP (TLE3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22094-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TLE3 Polyclonal antibody (22094-1-AP)

    IHC staining of human lung cancer using 22094-1-AP (same clone as 22094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22094-1-AP (TLE3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22094-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TLE3 Polyclonal antibody (22094-1-AP)

    IF Staining of HepG2 using 22094-1-AP (same clone as 22094-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TLE3 antibody (22094-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 22094-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22094-1-PBS targets TLE3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TLE3 fusion protein Ag17300 相同性解析による交差性が予測される生物種
Full Name transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
Calculated molecular weight 772 aa, 83 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC015729
Gene symbol TLE3
Gene ID (NCBI) 7090
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Transducin-like enhancer of split 3(TLE3) is a corepressor that binds to a variant of transciption factor, which mediated by CTNNB1 and TCF family members in Wnt signaling. It's suggested that effect of full-length TLE family members be regulated by association with dominant-negative AES.