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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TLE4 Polyclonal antibody (30959-1-AP)

    WB analysis using 30959-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30959-1-AP (TLE4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using TLE4 Polyclonal antibody (30959-1-AP)

    IP experiment of K-562 using 30959-1-AP

    IP result of anti-TLE4 (IP:30959-1-AP, 4ug; Detection:30959-1-AP 1:800) with K-562 cells lysate 1720 ug.

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TLE4 Polyclonal antibody

TLE4 Polyclonal Antibody for WB, IP, ELISA
Cat No. 30959-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

GRG4, KIAA1261

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, Neuro-2a cells
Positive IP detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30959-1-AP targets TLE4 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TLE4 fusion protein Ag34006 相同性解析による交差性が予測される生物種
Full Name transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila)
Calculated molecular weight 84 kDa
Observed molecular weight76-88 kDa
GenBank accession numberBC059405
Gene Symbol TLE4
Gene ID (NCBI) 7091
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ04727
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TLE4 (Transducin-like enhancer protein 4), also known as GRG4. It is predicted to be located in the nucleus. Tle4 promotes acquisition of corticothalamic identity and blocks emergence of core characteristics of subcerebral/corticospinal projection neuron identity, including gene expression and connectivity. During the first post-natal week, when corticothalamic innervation is ongoing, Tle4 is required to stabilize corticothalamic neuron identity, limiting interference from differentiation programs of developmentally related neuron classes (PMID: 37561632). The molecular weight of TLE4 is 76 kDa.

Protocols

Product Specific Protocols
WB protocol for TLE4 antibody 30959-1-APDownload protocol
IP protocol for TLE4 antibody 30959-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols