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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human TLR3/CD283 Rabbit Recombinant Antibody, PBS Only

TLR3/CD283 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

230445F1

Cat no : 98016-1-PBS

Synonyms

TLR3, Toll-like receptor 3, toll like receptor 3, CD283, 230445F1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human monocyte-derived immature dendritic cells using Anti-Human TLR3/CD283 Rabbit Recombinant Antibody (98016-1-RR)

    FC experiment of human monocyte-derived immature dendritic cells using 98016-1-RR (same clone as 98016-1-PBS)

    1x10^6 human monocyte-derived immature dendritic cells were intracellularly stained with 0.25 ug Anti-Human TLR3/CD283 Rabbit Recombinant Antibody (98016-1-RR, Clone: 230445F1) or 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with APC anti-human CD11b. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98016-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human monocyte-derived immature dendritic cells using Anti-Human TLR3/CD283 Rabbit Recombinant Antibody (98016-1-RR)

    FC experiment of human monocyte-derived immature dendritic cells using 98016-1-RR (same clone as 98016-1-PBS)

    1x10^6 human monocyte-derived immature dendritic cells were intracellularly stained with 0.25 ug Anti-Human TLR3/CD283 Rabbit Recombinant Antibody (98016-1-RR, Clone: 230445F1) (red) or 0.25 ug Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98016-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98016-1-RR

    Affinity and Kinetic Characterization of 98016-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98016-1-RR against Human TLR3/CD283 were performed. The affinity constant is 5.56 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 98016-1-PBS is TLR3/CD283 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TLR3/CD283 fusion protein Ag34044 相同性解析による交差性が予測される生物種
Full Name toll-like receptor 3
Calculated molecular weight 904 aa, 104 kDa
GenBank accession numberBC094737
Gene symbol TLR3
Gene ID (NCBI) 7098
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TLR3 belongs to the Toll-like receptor family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR that is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production, and dendritic cell (DC) maturation (PMID: 18262679).