Validation Data Gallery
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Tested Applications
| Positive FC (Intra) detected in | human monocyte-derived immature dendritic cells |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-98016-2 targets TLR3/CD283 in FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | TLR3/CD283 fusion protein Ag34044 相同性解析による交差性が予測される生物種 |
| Full Name | toll-like receptor 3 |
| Calculated molecular weight | 904 aa, 104 kDa |
| GenBank accession number | BC094737 |
| Gene Symbol | TLR3 |
| Gene ID (NCBI) | 7098 |
| RRID | AB_3673537 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O15455 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
TLR3 belongs to the Toll-like receptor family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR that is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production, and dendritic cell (DC) maturation (PMID: 18262679).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL594 TLR3/CD283 antibody CL594-98016-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |