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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TLR4 Monoclonal antibody, PBS Only

TLR4 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3G9A4

Cat no : 66350-1-PBS

Synonyms

ARMD10, CD284, hToll, TLR4, TOLL, toll like receptor 4

Validation Data Gallery

Applications:
  • WB analysis of RAW 264.7 using 66350-1-Ig (same clone as 66350-1-PBS)

    WB analysis of RAW 264.7 using 66350-1-Ig (same clone as 66350-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66350-1-Ig (TLR4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 66350-1-Ig (same clone as 66350-1-PBS)

    WB analysis of NIH/3T3 using 66350-1-Ig (same clone as 66350-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66350-1-Ig (TLR4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

  • WB analysis of pig liver using 66350-1-Ig (same clone as 66350-1-PBS)

    WB analysis of pig liver using 66350-1-Ig (same clone as 66350-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66350-1-Ig (TLR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

  • WB analysis of rat liver using 66350-1-Ig (same clone as 66350-1-PBS)

    WB analysis of rat liver using 66350-1-Ig (same clone as 66350-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 66350-1-Ig (TLR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66350-1-Ig (same clone as 66350-1-PBS)

    IHC staining of human tonsillitis using 66350-1-Ig (same clone as 66350-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66350-1-Ig (TLR4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66350-1-Ig (same clone as 66350-1-PBS)

    IHC staining of human tonsillitis using 66350-1-Ig (same clone as 66350-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66350-1-Ig (TLR4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

66350-1-PBS targets TLR4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TLR4 fusion protein Ag13857 相同性解析による交差性が予測される生物種
Full Name toll-like receptor 4
Calculated molecular weight 839 aa, 96 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC117422
Gene symbol TLR4
Gene ID (NCBI) 7099
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TLR4, also named as CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.