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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TLR6 Recombinant antibody

TLR6 Recombinant Antibody for IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

241926F2

Cat no : 84811-2-RR

Synonyms

Toll-like receptor 6, toll like receptor 6, CD286, 241926F2

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using TLR6 Recombinant antibody (84811-2-RR)

    IF Staining of mouse spleen using 84811-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using TLR6 antibody (84811-2-RR, Clone: 241926F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using TLR6 Recombinant antibody (84811-2-RR)

    IF Staining of mouse spleen using 84811-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using TLR6 antibody (84811-2-RR, Clone: 241926F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IF-P detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84811-2-RR targets TLR6 in IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name toll-like receptor 6
Calculated molecular weight 480 aa, 56 kDa
GenBank accession numberBC111755
Gene symbol TLR6
Gene ID (NCBI) 10333
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TLR6, also known as CD286, belongs to the Toll-like receptor (TLR) family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR6 can form a heterodimer with TLR2. TLR6 and TLR2 are both recruited to the macrophage phagosome, where they recognize peptidoglycan, a Gram-positive pathogen component (PMID: 11095740). TLR6 interacts with CD36, following CD36 stimulation by oxLDL or amyloid-beta 42, and forms a heterodimer with TLR4. The trimeric complex is internalized and triggers an inflammatory response.

Protocols

Product Specific Protocols
IF protocol for TLR6 antibody 84811-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols