Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:300-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20718-1-AP targets TMC1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | transmembrane channel-like 1 |
| Calculated molecular weight | 88 kDa |
| Observed molecular weight | 88 kDa |
| GenBank accession number | NM_138691 |
| Gene Symbol | TMC1 |
| Gene ID (NCBI) | 117531 |
| RRID | AB_10693676 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TDI8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Transmembrane channel-like protein 1 (TMC1), is an integral membrane protein with six transmembrane domains and cytoplasmically oriented N- and C- termini (PMID: 20672865). Human TMC1 and mouse Tmc1 mRNA expression were detected in human fetal cochlea and inner-ear tissue of postnatal mouse, respectively. TMC1 is required for cochlear hair-cell function (PMID: 11850618). It is a component of hair cell transduction channels and contributes to permeation properties (PMID: 23871232). Mutations in TMC1 gene can cause progressive postlingual hearing loss and profound prelingual deafness (PMID: 11850618). The antibody is specific to TMC1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TMC1 antibody 20718-1-AP | Download protocol |
| IHC protocol for TMC1 antibody 20718-1-AP | Download protocol |
| IF protocol for TMC1 antibody 20718-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |