TMED2 Polyclonal antibody

Name: TMED2 Polyclonal antibody
Brand: Proteintech
SKU: 11981-1-AP

TMED2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11981-1-AP

Synonyms

Membrane protein p24A, P24A, RNP24, TMED2

Validation Data Gallery

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AllWBIHC

WB analysis using 11981-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11981-1-AP (TMED2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11981-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11981-1-AP (TMED2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse skin using 11981-1-AP
mouse skin tissue were subjected to SDS PAGE followed by western blot with 11981-1-AP (TMED2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 11981-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11981-1-AP (TMED2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 11981-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11981-1-AP (TMED2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas cancer using 11981-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11981-1-AP (TMED2 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas cancer using 11981-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11981-1-AP (TMED2 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human pancreas cancer using 11981-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11981-1-AP (TMED2 antibody) at dilution of 1:50 (under 10x lens).

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Cat No. 11981-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, mouse pancreas tissue, mouse skin tissue, mouse liver tissue, HeLa cells, rat kidney tissue
Positive IHC detected in	human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 7 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

11981-1-AP targets TMED2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TMED2 fusion protein Ag2607 相同性解析による交差性が予測される生物種
Full Name	transmembrane emp24 domain trafficking protein 2
Calculated molecular weight	201 aa, 23 kDa
Observed molecular weight	20-23 kDa
GenBank accession number	BC025957
Gene symbol	TMED2
Gene ID (NCBI)	10959
RRID	AB_2204353
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TMED2 antibody 11981-1-AP	Download protocol
IHC protocol for TMED2 antibody 11981-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Res A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis. Authors - Shulin Li View Article
human	WB	BMC Biol ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier. Authors - Xihua Yue View Article
human	WB,IF	BMC Cancer The circular RNA CDR1as regulate cell proliferation via TMED2 and TMED10. Authors - Xue Yang View Article
human	WB	Adv Sci (Weinh) TMED3 Complex Mediates ER Stress-Associated Secretion of CFTR, Pendrin, and SARS-CoV-2 Spike. Authors - Hak Park View Article
human	WB	Ann Transl Med Screening of the novel immune-suppressive biomarkers of TMED family and whether knockdown of TMED2/3/4/9 inhibits cell migration and invasion in breast cancer Authors - Zheng Fang KD Validated View Article
human	WB	Curr Med Sci TMED2 Induces Cisplatin Resistance in Breast Cancer via Targeting the KEAP1-Nrf2 Pathway Authors - Chen Liang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMED2 Polyclonal antibody

TMED2 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 11981-1-AP

WB analysis of mouse liver using 11981-1-AP

WB analysis of mouse skin using 11981-1-AP

WB analysis of mouse pancreas using 11981-1-AP

WB analysis of mouse pancreas using 11981-1-AP

IHC staining of human pancreas cancer using 11981-1-AP

IHC staining of human pancreas cancer using 11981-1-AP

IHC staining of human pancreas cancer using 11981-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.

ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier.

The circular RNA CDR1as regulate cell proliferation via TMED2 and TMED10.

TMED3 Complex Mediates ER Stress-Associated Secretion of CFTR, Pendrin, and SARS-CoV-2 Spike.

Screening of the novel immune-suppressive biomarkers of TMED family and whether knockdown of TMED2/3/4/9 inhibits cell migration and invasion in breast cancer

TMED2 Induces Cisplatin Resistance in Breast Cancer via Targeting the KEAP1-Nrf2 Pathway