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KD/KO Validated

TMED3 Polyclonal antibody

Name: TMED3 Polyclonal antibody
Brand: Proteintech
SKU: 21902-1-AP

TMED3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 21902-1-AP

Synonyms

Transmembrane emp24 domain-containing protein 3, p26, p24gamma4, p24 family protein gamma-4, Membrane protein p24B

Validation Data Gallery

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AllWBIHC

WB analysis using 21902-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21902-1-AP (TMED3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 21902-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 21902-1-AP (TMED3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 21902-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 21902-1-AP (TMED3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse small intestine using 21902-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21902-1-AP (TMED3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skin using 21902-1-AP
mouse skin tissue were subjected to SDS PAGE followed by western blot with 21902-1-AP (TMED3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21902-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21902-1-AP (TMED3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human stomach cancer using 21902-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21902-1-AP (TMED3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 21902-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, MCF-7 cells, mouse small intestine tissue, mouse skin tissue, HT-1080 cells, HepG2 cells
Positive IHC detected in	human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 6 publications below

Product Information

21902-1-AP targets TMED3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TMED3 fusion protein Ag14032 相同性解析による交差性が予測される生物種
Full Name	transmembrane emp24 protein transport domain containing 3
Calculated molecular weight	217 aa, 25 kDa
Observed molecular weight	21-25 kDa
GenBank accession number	BC000027
Gene symbol	TMED3
Gene ID (NCBI)	23423
RRID	AB_10897159
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TMED3 antibody 21902-1-AP	Download protocol
IHC protocol for TMED3 antibody 21902-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Clin Invest Chromosomal 3q amplicon encodes essential regulators of secretory vesicles that drive secretory addiction in cancer Authors - Xiaochao Tan View Article
human	WB	J Biol Chem Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stress. Authors - Chengfeng Merriman View Article
human	WB	Toxicol Appl Pharmacol TMED3 exerts a protumor function in non-small cell lung cancer by enhancing the Wnt/β-catenin pathway via regulation of AKT. Authors - Danjie Zhang KD Validated View Article
human	WB	Onco Targets Ther TMED3 Promotes Proliferation and Migration in Breast Cancer Cells by Activating Wnt/β-Catenin Signaling. Authors - Xiumei Zhang KD Validated View Article
human	WB	Ann Transl Med Screening of the novel immune-suppressive biomarkers of TMED family and whether knockdown of TMED2/3/4/9 inhibits cell migration and invasion in breast cancer Authors - Zheng Fang KD Validated View Article
human	WB	J Cancer Res Clin Oncol Analysis and Validation of TMED3 correlates with poor prognosis and tumor immune infiltration of glioma Authors - Gang Liao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMED3 Polyclonal antibody

TMED3 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 21902-1-AP

WB analysis of HT-1080 using 21902-1-AP

WB analysis of MCF-7 using 21902-1-AP

WB analysis of mouse small intestine using 21902-1-AP

WB analysis of mouse skin using 21902-1-AP

WB analysis of HeLa using 21902-1-AP

IHC staining of human stomach cancer using 21902-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Chromosomal 3q amplicon encodes essential regulators of secretory vesicles that drive secretory addiction in cancer

Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stress.

TMED3 exerts a protumor function in non-small cell lung cancer by enhancing the Wnt/β-catenin pathway via regulation of AKT.

TMED3 Promotes Proliferation and Migration in Breast Cancer Cells by Activating Wnt/β-Catenin Signaling.

Screening of the novel immune-suppressive biomarkers of TMED family and whether knockdown of TMED2/3/4/9 inhibits cell migration and invasion in breast cancer

Analysis and Validation of TMED3 correlates with poor prognosis and tumor immune infiltration of glioma