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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

TMED4 Polyclonal antibody

TMED4 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14141-1-AP

Synonyms

Putative NF-kappa-B-activating protein 156, p24alpha3, p24 family protein alpha-3, GMP25iso, ERS25

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMED4 Polyclonal antibody (14141-1-AP)

    WB analysis using 14141-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14141-1-AP (TMED4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using TMED4 Polyclonal antibody (14141-1-AP)

    WB analysis of mouse liver using 14141-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14141-1-AP (TMED4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TMED4 Polyclonal antibody (14141-1-AP)

    WB analysis of HepG2 using 14141-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14141-1-AP (TMED4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using TMED4 Polyclonal antibody (14141-1-AP)

    IP experiment of mouse liver using 14141-1-AP

    IP result of anti-TMED4 (IP:14141-1-AP, 4ug; Detection:14141-1-AP 1:700) with mouse liver tissue lysate 6000 ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TMED4 Polyclonal antibody (14141-1-AP)

    IF Staining of HepG2 using 14141-1-AP

    Immunofluorescent analysis of HepG2 cells using 14141-1-AP (TMED4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse liver tissue, HepG2 cells, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14141-1-AP targets TMED4 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMED4 fusion protein Ag5234 相同性解析による交差性が予測される生物種
Full Name transmembrane emp24 protein transport domain containing 4
Calculated molecular weight 227 aa, 26 kDa
Observed molecular weight 24-26 kDa
GenBank accession numberBC057851
Gene symbol TMED4
Gene ID (NCBI) 222068
RRIDAB_10642696
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TMED4 antibody 14141-1-APDownload protocol
IF protocol for TMED4 antibody 14141-1-APDownload protocol
IP protocol for TMED4 antibody 14141-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.

Authors - Shulin Li
  • KD Validated
View Article
humanWB

Ann Transl Med

Screening of the novel immune-suppressive biomarkers of TMED family and whether knockdown of TMED2/3/4/9 inhibits cell migration and invasion in breast cancer

Authors - Zheng Fang
  • KD Validated
View Article