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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMEFF2 Polyclonal antibody

TMEFF2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11928-1-AP

Synonyms

HPP1, TENB2, TMEFF2, Tomoregulin 2, TPEF, TR, TR 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEFF2 Polyclonal antibody (11928-1-AP)

    WB analysis using 11928-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11928-1-AP (TMEFF2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using TMEFF2 Polyclonal antibody (11928-1-AP)

    WB analysis of human brain using 11928-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11928-1-AP (TMEFF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TMEFF2 Polyclonal antibody (11928-1-AP)

    WB analysis of HeLa using 11928-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11928-1-AP (TMEFF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using TMEFF2 Polyclonal antibody (11928-1-AP)

    WB analysis of PC-3 using 11928-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 11928-1-AP (TMEFF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TMEFF2 Polyclonal antibody (11928-1-AP)

    IHC staining of human prostate cancer using 11928-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11928-1-AP (TMEFF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TMEFF2 Polyclonal antibody (11928-1-AP)

    IHC staining of human prostate cancer using 11928-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11928-1-AP (TMEFF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inSH-SY5Y cells, HeLa cells, human brain tissue, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11928-1-AP targets TMEFF2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEFF2 fusion protein Ag2609 相同性解析による交差性が予測される生物種
Full Name transmembrane protein with EGF-like and two follistatin-like domains 2
Calculated molecular weight 374 aa, 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC008973
Gene symbol TMEFF2
Gene ID (NCBI) 23671
RRIDAB_2204477
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TMEFF2 antibody 11928-1-APDownload protocol
IHC protocol for TMEFF2 antibody 11928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Immunol

Gene expression profiling and real-time PCR analyses identify novel potential cancer-testis antigens in multiple myeloma.

Authors - Condomines Maud M
View Article
humanIHC

Cancer Cell Int

TMEFF2 promoter hypermethylation is an unfavorable prognostic marker in gliomas.

Authors - Sidi Xie
View Article