TMEM100 Polyclonal antibody

Name: TMEM100 Polyclonal antibody
Brand: Proteintech
SKU: 25581-1-AP

TMEM100 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 25581-1-AP

Synonyms

Transmembrane protein 100

Validation Data Gallery

Filter:

AllIHC

IHC staining of human lung using 25581-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 25581-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 25581-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 25581-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 25581-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25581-1-AP (TMEM100 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human stomach cancer using 25581-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25581-1-AP (TMEM100 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse brain using 25581-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 25581-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 25581-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 25581-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 25581-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected in

mouse brain tissue, human brain tissue, human lung tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

25581-1-AP targets TMEM100 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TMEM100 fusion protein Ag22272 相同性解析による交差性が予測される生物種
Full Name	transmembrane protein 100
Calculated molecular weight	134 aa, 14 kDa
GenBank accession number	BC010128
Gene symbol	TMEM100
Gene ID (NCBI)	55273
RRID	AB_2880138
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for TMEM100 antibody 25581-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Biomolecules Identification of a Fibroblast-Related Prognostic Model in Glioma Based on Bioinformatics Methods Authors - Haofuzi Zhang View Article
human	WB	World J Clin Oncol Regulation of TMEM100 expression by epigenetic modification, effects on proliferation and invasion of esophageal squamous carcinoma Authors - Yue-Feng Xu View Article
human	IF	Front Cell Neurosci A novel histone deacetylase inhibitor W2A-16 improves the barrier integrity in brain vascular endothelial cells Authors - Yasuteru Inoue View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMEM100 Polyclonal antibody

TMEM100 Polyclonal Antibody for IHC, ELISA

Validation Data Gallery

IHC staining of human lung using 25581-1-AP

IHC staining of human lung using 25581-1-AP

IHC staining of human brain using 25581-1-AP

IHC staining of human brain using 25581-1-AP

IHC staining of human stomach cancer using 25581-1-AP

IHC staining of human stomach cancer using 25581-1-AP

IHC staining of mouse brain using 25581-1-AP

IHC staining of mouse brain using 25581-1-AP

IHC staining of human stomach cancer using 25581-1-AP

IHC staining of human stomach cancer using 25581-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Identification of a Fibroblast-Related Prognostic Model in Glioma Based on Bioinformatics Methods

Regulation of TMEM100 expression by epigenetic modification, effects on proliferation and invasion of esophageal squamous carcinoma

A novel histone deacetylase inhibitor W2A-16 improves the barrier integrity in brain vascular endothelial cells