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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human lung tissue using TMEM100 Polyclonal antibody (25581-1-AP)

    IHC staining of human lung using 25581-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using TMEM100 Polyclonal antibody (25581-1-AP)

    IHC staining of human lung using 25581-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using TMEM100 Polyclonal antibody (25581-1-AP)

    IHC staining of human brain using 25581-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using TMEM100 Polyclonal antibody (25581-1-AP)

    IHC staining of human brain using 25581-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TMEM100 Polyclonal antibody (25581-1-AP)

    IHC staining of human stomach cancer using 25581-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TMEM100 Polyclonal antibody (25581-1-AP)

    IHC staining of human stomach cancer using 25581-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TMEM100 Polyclonal antibody (25581-1-AP)

    IHC staining of human stomach cancer using 25581-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25581-1-AP (TMEM100 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TMEM100 Polyclonal antibody (25581-1-AP)

    IHC staining of human stomach cancer using 25581-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25581-1-AP (TMEM100 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TMEM100 Polyclonal antibody (25581-1-AP)

    IHC staining of mouse brain using 25581-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TMEM100 Polyclonal antibody (25581-1-AP)

    IHC staining of mouse brain using 25581-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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TMEM100 Polyclonal antibody

TMEM100 Polyclonal Antibody for IHC, ELISA
Cat No. 25581-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

Transmembrane protein 100

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse brain tissue, human brain tissue, human lung tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25581-1-AP targets TMEM100 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM100 fusion protein Ag22272 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 100
Calculated molecular weight 134 aa, 14 kDa
GenBank accession numberBC010128
Gene Symbol TMEM100
Gene ID (NCBI) 55273
RRIDAB_2880138
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NV29
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for TMEM100 antibody 25581-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Biomolecules

Identification of a Fibroblast-Related Prognostic Model in Glioma Based on Bioinformatics Methods

Authors - Haofuzi Zhang
View Article
humanWB

World J Clin Oncol

Regulation of TMEM100 expression by epigenetic modification, effects on proliferation and invasion of esophageal squamous carcinoma

Authors - Yue-Feng Xu
View Article
humanIF

Front Cell Neurosci

A novel histone deacetylase inhibitor W2A-16 improves the barrier integrity in brain vascular endothelial cells

Authors - Yasuteru Inoue
View Article