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Target

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Species Reactivity

Species Reactivity

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Conjugate

  • Featured Product
  • KD/KO Validated

TMEM102 Polyclonal antibody

TMEM102 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25505-1-AP

Synonyms

CBAP, TMEM102, transmembrane protein 102

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using TMEM102 Polyclonal antibody (25505-1-AP)

    WB analysis of HepG2 using 25505-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25505-1-AP (TMEM102 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TMEM102 Polyclonal antibody (25505-1-AP)

    WB analysis of HeLa using 25505-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25505-1-AP (TMEM102 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using TMEM102 Polyclonal antibody (25505-1-AP)

    WB analysis of mouse brain using 25505-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25505-1-AP (TMEM102 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using TMEM102 Polyclonal antibody (25505-1-AP)

    WB analysis of Jurkat using 25505-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25505-1-AP (TMEM102 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using TMEM102 Polyclonal antibody (25505-1-AP)

    IHC staining of human testis using 25505-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25505-1-AP (TMEM102 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using TMEM102 Polyclonal antibody (25505-1-AP)

    IHC staining of human testis using 25505-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25505-1-AP (TMEM102 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using TMEM102 Polyclonal antibody (25505-1-AP)

    IHC staining of human heart using 25505-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25505-1-AP (TMEM102 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using TMEM102 Polyclonal antibody (25505-1-AP)

    IHC staining of human heart using 25505-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25505-1-AP (TMEM102 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, Jurkat cells, mouse brain tissue
Positive IHC detected inhuman testis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25505-1-AP targets TMEM102 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM102 fusion protein Ag22213 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 102
Calculated molecular weight 508 aa, 54 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC046189
Gene symbol TMEM102
Gene ID (NCBI) 284114
RRIDAB_2880107
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TMEM102 antibody 25505-1-APDownload protocol
IHC protocol for TMEM102 antibody 25505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Biol Chem

CBAP regulates the function of Akt-associated TSC protein complexes to modulate mTORC1 signaling

Authors - Wei-Ting Liao
  • KD Validated
View Article