Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 67205-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67205-1-Ig (TMEM111 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 67205-1-Ig
WB result of TMEM111 antibody (67205-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM111 transfected HEK-293 cells.
WB analysis of mouse heart using 67205-1-Ig
mouse heart tissue were subjected to SDS PAGE followed by western blot with 67205-1-Ig (TMEM111 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 67205-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67205-1-Ig (TMEM111 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 67205-1-Ig
human heart tissue were subjected to SDS PAGE followed by western blot with 67205-1-Ig (TMEM111 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of pig heart using 67205-1-Ig
pig heart tissue were subjected to SDS PAGE followed by western blot with 67205-1-Ig (TMEM111 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 67205-1-Ig
rat heart tissue were subjected to SDS PAGE followed by western blot with 67205-1-Ig (TMEM111 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of mouse lung using 67205-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67205-1-Ig (TMEM111 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 67205-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67205-1-Ig (TMEM111 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A431 using 67205-1-Ig
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using TMEM111 antibody (67205-1-Ig, Clone: 2F8G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

Featured Product
KD/KO Validated

TMEM111 Monoclonal antibody

TMEM111 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67205-1-Ig

Clone No.2F8G5

Publications(6)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EMC3, POB, TMEM111, transmembrane protein 111

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, human heart tissue, pig heart tissue, rat heart tissue, mouse heart tissue, HepG2 cells, Jurkat cells, HSC-T6, NIH/3T3 cells, 4T1 cells
Positive IHC detected in	mouse lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

67205-1-Ig targets TMEM111 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity	Human, mouse, rat, pig
Cited Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	TMEM111 fusion protein Ag18365 相同性解析による交差性が予測される生物種
Full Name	transmembrane protein 111
Calculated molecular weight	261 aa, 30 kDa
Observed molecular weight	30 kDa
GenBank accession number	BC022807
Gene Symbol	TMEM111
Gene ID (NCBI)	55831
RRID	AB_2882498
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9P0I2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TMEM111,also names as EMC3, is part of the endoplasmic reticulum-associated secretory pathway (PMID: 19797678). TMEM111 was required for murine pulmonary surfactant synthesis and lung function at birth (PMID: 29083321). It can be detect a band of 30 kDa.

Protocols

Product Specific Protocols
WB protocol for TMEM111 antibody 67205-1-Ig	Download protocol
IHC protocol for TMEM111 antibody 67205-1-Ig	Download protocol
IF protocol for TMEM111 antibody 67205-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	IP	Mol Cell WNK1 is an assembly factor for the human ER membrane protein complex. Authors - Tino Pleiner View Article
human	WB	J Cell Biol A selectivity filter in the ER membrane protein complex limits protein misinsertion at the ER Authors - Tino Pleiner View Article
human	WB	bioRxiv Role of a holo-insertase complex in the biogenesis of biophysically diverse ER membrane proteins Authors - Katharine R Page View Article
human	IF	EMBO J The ER membrane protein complex restricts mitophagy by controlling BNIP3 turnover Authors - Jose M Delgado KO Validated View Article
human	WB	Science MTCH2 is a mitochondrial outer membrane protein insertase Authors - Alina Guna View Article
human	IF	bioRxiv The ER membrane protein complex governs lysosomal turnover of a mitochondrial tail-anchored protein, BNIP3, to restrict mitophagy Authors - Jose M Delgado View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 67205-1-Ig

WB analysis of HEK-293 using 67205-1-Ig

WB analysis of mouse heart using 67205-1-Ig

WB analysis using 67205-1-Ig

WB analysis of human heart using 67205-1-Ig

WB analysis of pig heart using 67205-1-Ig

WB analysis of rat heart using 67205-1-Ig

IHC staining of mouse lung using 67205-1-Ig

IHC staining of mouse lung using 67205-1-Ig

IF Staining of A431 using 67205-1-Ig

TMEM111 Monoclonal antibody

TMEM111 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

WNK1 is an assembly factor for the human ER membrane protein complex.

A selectivity filter in the ER membrane protein complex limits protein misinsertion at the ER

Role of a holo-insertase complex in the biogenesis of biophysically diverse ER membrane proteins

The ER membrane protein complex restricts mitophagy by controlling BNIP3 turnover

MTCH2 is a mitochondrial outer membrane protein insertase

The ER membrane protein complex governs lysosomal turnover of a mitochondrial tail-anchored protein, BNIP3, to restrict mitophagy