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  • Featured Product
  • KD/KO Validated

TMEM111 Monoclonal antibody

TMEM111 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67205-1-Ig
Clone No.2F8G5

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

EMC3, POB, TMEM111, transmembrane protein 111

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, human heart tissue, pig heart tissue, rat heart tissue, mouse heart tissue, HepG2 cells, Jurkat cells, HSC-T6, NIH/3T3 cells, 4T1 cells
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67205-1-Ig targets TMEM111 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TMEM111 fusion protein Ag18365 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 111
Calculated molecular weight 261 aa, 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC022807
Gene Symbol TMEM111
Gene ID (NCBI) 55831
RRIDAB_2882498
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9P0I2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMEM111,also names as EMC3, is part of the endoplasmic reticulum-associated secretory pathway (PMID: 19797678). TMEM111 was required for murine pulmonary surfactant synthesis and lung function at birth (PMID: 29083321). It can be detect a band of 30 kDa.

Protocols

Product Specific Protocols
WB protocol for TMEM111 antibody 67205-1-IgDownload protocol
IHC protocol for TMEM111 antibody 67205-1-IgDownload protocol
IF protocol for TMEM111 antibody 67205-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IP

Mol Cell

WNK1 is an assembly factor for the human ER membrane protein complex.

Authors - Tino Pleiner
humanWB

J Cell Biol

A selectivity filter in the ER membrane protein complex limits protein misinsertion at the ER

Authors - Tino Pleiner
humanWB

bioRxiv

Role of a holo-insertase complex in the biogenesis of biophysically diverse ER membrane proteins

Authors - Katharine R Page
humanIF

EMBO J

The ER membrane protein complex restricts mitophagy by controlling BNIP3 turnover

Authors - Jose M Delgado
  • KO Validated
humanWB

Science

MTCH2 is a mitochondrial outer membrane protein insertase

Authors - Alina Guna
humanIF

bioRxiv

The ER membrane protein complex governs lysosomal turnover of a mitochondrial tail-anchored protein, BNIP3, to restrict mitophagy

Authors - Jose M Delgado
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