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Validation Data Gallery

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  • Western Blot (WB) analysis of U2OS cells using TMEM115 Polyclonal antibody (25536-1-AP)

    WB analysis of U2OS using 25536-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 25536-1-AP (TMEM115 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TMEM115 Polyclonal antibody (25536-1-AP)

    WB analysis using 25536-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25536-1-AP (TMEM115 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using TMEM115 Polyclonal antibody (25536-1-AP)

    IHC staining of human ovary cancer using 25536-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25536-1-AP (TMEM115 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using TMEM115 Polyclonal antibody (25536-1-AP)

    IHC staining of human ovary cancer using 25536-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25536-1-AP (TMEM115 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using TMEM115 Polyclonal antibody (25536-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 25536-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25536-1-AP (TMEM115 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using TMEM115 Polyclonal antibody (25536-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 25536-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25536-1-AP (TMEM115 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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TMEM115 Polyclonal antibody

TMEM115 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25536-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Transmembrane protein 115, Placental protein 6, PL6, LUCA11.2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU2OS cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inhuman ovary cancer tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25536-1-AP targets TMEM115 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM115 fusion protein Ag22258 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 115
Calculated molecular weight 351 aa, 38 kDa
Observed molecular weight35-38 kDa
GenBank accession numberBC017367
Gene Symbol TMEM115
Gene ID (NCBI) 11070
RRIDAB_3669482
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12893
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMEM115 also known as PL6, located on chromosome 3p21.3, was originally thought to function as a tumor suppressor. TMEM115 is an integral membrane protein of the Golgi complex involved in retrograde transport.

Protocols

Product Specific Protocols
WB protocol for TMEM115 antibody 25536-1-APDownload protocol
IHC protocol for TMEM115 antibody 25536-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols