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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMEM119 Recombinant antibody, PBS Only

TMEM119 Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240433F2

Cat no : 83448-6-PBS

Synonyms

UNQ731/PRO1415, transmembrane protein 119, Osteoblast induction factor, OBIF, 240433F2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM119 Recombinant antibody (83448-6-RR)

    WB analysis using 83448-6-RR (same clone as 83448-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83448-6-RR (TMEM119 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83448-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83448-6-RR

    Affinity and Kinetic Characterization of 83448-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83448-6-RR against Human TMEM119 were performed. The affinity constant is 9.33 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83448-6-PBS is TMEM119 Fusion Protein expressed in E. coli.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 119
Calculated molecular weight29 kDa
Observed molecular weight52 kDa
GenBank accession numberNM_181724.2
Gene symbol TMEM119
Gene ID (NCBI) 338773
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TMEM119 immunohistochemistry might provide a useful tool for investigating the biology and pathology of human microglia(PMID: 26250788). Microglia can be detected clearly using Catalog#66948-1-Ig. The predicted MW of TMEM119 is 29 kDa. Catalog#66948-1-Ig recognizes 50 kDa band may due to glycosylation.