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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMEM119 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 78.1-5000 pg/mL

Cat No : MP00444-4

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00444-4, Human TMEM119 Recombinant Matched Antibody Pair - PBS only. 83448-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0796. 83448-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

    Sandwich ELISA standard curve of MP00444-4

    Sandwich ELISA standard curve of MP00444-4, Human TMEM119 Recombinant Matched Antibody Pair - PBS only. 83448-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0796. 83448-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • The mean TMEM119 concentration was determined to be 2,998.4 pg/mL in HEK-293 cell extract based on a 1.5 mg/mL extract load and 3,201.6 pg/mL in SH-SY5Y cell extract based on a 1.2 mg/mL extract load.

    Sample test of MP00444-4

    The mean TMEM119 concentration was determined to be 2,998.4 pg/mL in HEK-293 cell extract based on a 1.5 mg/mL extract load and 3,201.6 pg/mL in SH-SY5Y cell extract based on a 1.2 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00444-4 targets TMEM119 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 78.1-5000 pg/mL (Sandwich ELISA);

Capture antibody: TMEM119 Recombinant antibody, PBS Only (Capture/Detector) 83448-4-PBS (240433C12). 100 μg. Concentration 1 mgl/ml.

Detection antibody: TMEM119 Recombinant antibody, PBS Only (Detector) 83448-3-PBS (240433D12). 100 μg. Concentration 1 mgl/ml.

Alternative TMEM119 matched antibody pairs: MP00444-1, MP00444-2, MP00444-3

Matched pair protein standard: Eg0796

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83448-4-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name transmembrane protein 119
Synonyms UNQ731/PRO1415, transmembrane protein 119, Osteoblast induction factor, OBIF, 240433C12
GenBank accession numberNM_181724.2
Gene symbol TMEM119
UniprotQ4V9L6
Gene ID (NCBI) 338773
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83448-3-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name transmembrane protein 119
Synonyms UNQ731/PRO1415, transmembrane protein 119, Osteoblast induction factor, OBIF, 240433D12
GenBank accession numberNM_181724.2
Gene symbol TMEM119
UniprotQ4V9L6
Gene ID (NCBI) 338773
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.