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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM126A Polyclonal antibody (20759-1-AP)

    WB analysis using 20759-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20759-1-AP (TMEM126A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TMEM126A Polyclonal antibody (20759-1-AP)

    IF Staining of HeLa using 20759-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMEM126A antibody (20759-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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TMEM126A Polyclonal antibody

TMEM126A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 20759-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

TMEM126, TMEM, OPA7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20759-1-AP targets TMEM126A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM126A fusion protein Ag14705 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 126A
Calculated molecular weight 195 aa, 22 kDa
Observed molecular weight20-25 kDa
GenBank accession numberBC007875
Gene Symbol TMEM126A
Gene ID (NCBI) 84233
RRIDAB_3669345
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H061
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMEM126A is a mitochondrial inner membrane protein that is enriched in the cristae. TMEM126A is necessary for Complex I assembly or stability.Complex I (CI) is the largest enzyme of the mitochondrial respiratory chain, and its defects are the main cause of mitochondrial disease.

Protocols

Product Specific Protocols
WB protocol for TMEM126A antibody 20759-1-APDownload protocol
IF protocol for TMEM126A antibody 20759-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols